Molecular Pathogens, 2025, Vol.16, No.6, 294-302 http://microbescipublisher.com/index.php/mp 300 So, to move towards a more stable and green agricultural approach, relying solely on "general microbial preparations" may be far from enough. The subsequent steps need to be more precise, such as customizing suitable microbial solutions based on the target wheat variety and the soil conditions of the local production area. Screening out reliable core microbiota is just the first step. Monitoring how they change in the rhizosphere and whether they can stabilize in the long term cannot be ignored. Furthermore, integrating microbiome management methods with traditional disease-resistant breeding approaches might be a more realistic breakthrough. Future research, in addition to optimizing at the technical application level, must also delve into the molecular level to further clarify how plants, microorganisms, and pathogens interact. At the same time, by leveraging high-throughput sequencing, network analysis, and other tools, we can guide us to achieve precise enrichment and friendly regulation of rhizosphere microorganisms in a true sense. Only in this way is it possible to achieve a crop management approach that is both disease-resistant and environmentally friendly. Acknowledgments The authors would like to thank all teachers and colleagues who provided guidance and assistance during this research, and for the peer review's revision suggestions. Conflict of Interest Disclosure The authors confirm that the study was conducted without any commercial or financial relationships and could be interpreted as a potential conflict of interest. References Boamah S., Zhang S., Xu B., Zhu N., and Li E., 2025, Trichoderma longibrachiatum TG1 colonization and signal pathway in alleviating salinity and Fusarium pseudograminearum stress in wheat, International Journal of Molecular Sciences, 26(9): 4018. https://doi.org/10.3390/ijms26094018 Bukhat S., Imran A., Javaid S., Shahid M., Majeed A., and Naqqash T., 2020, Communication of plants with microbial world: Exploring the regulatory networks for PGPR mediated defense signaling, Microbiological Research, 238: 126486. https://doi.org/10.1016/j.micres.2020.126486 Costa L., De Faria M., Chiaramonte J., Mendes L., Sepo E., De Hollander M., Fernandes J., Carrión V., Bettiol W., Mauchline T., Raaijmakers J., and Mendes R., 2023, Repeated exposure of wheat to the fungal root pathogen Bipolaris sorokiniana modulates Rhizosphere microbiome assembly and disease suppressiveness, Environmental Microbiome, 18: 85. https://doi.org/10.1186/s40793-023-00529-2 De Cássia Mesquita Da Cunha I., Da Silva A., Boleta E., Pellegrinetti T., Zagatto L., Zagatto S., De Chaves M., Mendes R., Patreze C., Tsai S., and Mendes L., 2024, The interplay between the inoculation of plant growth-promoting rhizobacteria and the Rhizosphere microbiome and their impact on plant phenotype, Microbiological Research, 283: 127706. https://doi.org/10.1016/j.micres.2024.127706 Dong Q., Duan D., Wang F., Yang K., Song Y., Wang Y., Wang D., Ji Z., Xu C., Jia P., Luan H., Guo S., Qi G., Mao K., Zhang X., Tian Y., Ma Y., and Ma F., 2024, The MdVQ37‐MdWRKY100 complex regulates salicylic acid content and MdRPM1 expression to modulate resistance to Glomerella leaf spot in apples, Plant Biotechnology Journal, 22(8): 2364-2376. https://doi.org/10.1111/pbi.14351 Francioli D., Kampouris I., Kuhl-Nagel T., Babin D., Sommermann L., Behr J., Chowdhury S., Zrenner R., Moradtalab N., Schloter M., Geistlinger J., Ludewig U., Neumann G., Smalla K., and Grosch R., 2025, Microbial inoculants modulate the rhizosphere microbiome alleviate plant stress responses and enhance maize growth at field scale, Genome Biology, 26(1): 148. https://doi.org/10.1186/s13059-025-03621-7 Ge J., Li D., Ding J., Xiao X., and Liang Y., 2023, Microbial coexistence in the rhizosphere and the promotion of plant stress resistance: a review, Environmental Research, 222: 115298. https://doi.org/10.1016/j.envres.2023.115298 Gu Y., Dong K., Geisen S., Yang W., Yan Y., Gu D., Liu N., Borisjuk N., Luo Y., and Friman V., 2020, The effect of microbial inoculant origin on the rhizosphere bacterial community composition and plant growth-promotion, Plant and Soil, 452: 105-117. https://doi.org/10.1007/s11104-020-04545-w Huang D.D., 2025, Role of mycorrhizal associations in wheat nutrition, Molecular Soil Biology, 16(3): 150-161. https://doi.org/10.5376/msb.2025.16.0015 Ji C., Chen Z., Kong X., Xin Z., Sun F., Xing J., Li C., Li K., Liang Z., and Cao H., 2022, Biocontrol and plant growth promotion by combined Bacillus spp., inoculation affecting pathogen and AMF communities in the wheat rhizosphere at low salt stress conditions, Frontiers in Plant Science, 13: 1043171. https://doi.org/10.3389/fpls.2022.1043171
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