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Screening of
Vibrio sp
., a Potential Pathogen in Marketed Sea Foods
5
The isolates were subjected to various ecological
factor such as various temperature (20
℃
, 30
℃
,
40
℃
, 50
℃
, 60
℃
), salinity range (5%, 10%, 15%,
20%, 25%), pH range (6,7,8,9,10), carbon (Glucose,
Sucrose, Lactose) and mineral source (CaCl
2
,
MgSO
4
, ZnSO
4
), that were analyzed at marketed
sea foods isolated vibrios. Apart from
Vibrio
cholerae
other
Vibrio sp.,
could not grown in 0%
NaCl, whereas all the isolates grew in 1% NaCl
(Table 4, 5).
Table 4 Effect of different pH on isolated Vibrio sp. growth from Marketed fish sample (The Values are measured in Optical Density)
Strain No.
Name of the
Vibrio
spp.
1
2
3
4
5
1
V. parahaemolyticus
0.02
0.29
0.38
0.32
0.04
2
V. parahaemolyticus
0.09
0.16
0.48
0.38
0.31
3
V. vulnificus
0.56
0.78
0.94
0.86
0.23
4
V. cholerae
,
0.24
0.45
0.84
0.73
0.29
5
V. alginolyticus
0.68
0.53
0.61
0.61
0.10
6
V. parahaemolyticus
0.05
0.42
0.37
0.13
0.18
7
V. parahaemolyticus
0.41
0.39
0.32
0.26
0.28
8
V. cholera
0.84
0.75
0.87
0.80
0.07
9
V. parahaemolyticus
0.24
0.57
0.87
0.29
0.29
10
V. mimicus.
0.02
0.439
0.58
0.56
1.06
11
V. vulnificus
0.53
0.7
0.76
0.79
0.12
12
V. parahaemolyticus
0.49
0.08
0.29
0.22
0.38
13
V. vulnificus
0.54
0.61
0.96
0.91
0.10
14
V. mimicus.
0.79
0.69
0.83
0.76
0.07
15
V. alginolyticus
0.72
0.23
0.16
0.91
0.52
16
V. mimicus.
0.35
0.25
0.26
0.39
0.29
17
V. mimicus.
0.73
0.76
0.84
0.80
1.09
18
V. parahaemolyticus
0.59
0.49
0.52
0.44
0.28
Table 5 Effect of different carbon sources on isolated Vibrio spp. growth from marketed fish sample (The Values are measured in
optical density)
Strain No
Name of the
Vibrio
sp.
Glucose
Sucrose
Lactose
1
V. parahaemolyticus
0.24
0.42
0.24
2
V. parahaemolyticus
0.23
0.15
0.43
3
V. vulnificus
0.48
0.16
0.24
4
V. cholerae
,
0.27
0.40
0.24
5
V. alginolyticus
0.19
0.36
0.8
6
V. parahaemolyticus
0.16
0.80
1.24
7
V. arahaemolyticus
0.42
0.36
0.30
8
V. cholerae
,
0.23
0.27
0.16
9
V. parahaemolyticus
0.19
0.42
0.24
10
V. mimicus
0.24
0.18
1.32
11
V. vulnificus
0.42
0.48
0.64
12
V. parahaemolyticus
1.35
0.78
0.90
13
V. vulnificus
0.36
0.30
0.31
14
V. mimicus
0.43
0.15
0.23
15
V. alginolyticus
0.10
0.80
0.24
16
V. mimicus
0.17
0.27
0.32
17
V. mimicus
0.43
0.63
0.47
18
V. parahaemolyticus
0.90
0.78
0.87