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Plant Gene and Trait 2012, Vol.3, No.6, 28
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33
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28
Research Report Open Access
QTLs Related to the Easy Curing Potential Mapped in Flue-cured Tobacco
Xiaolei Tan
1,2
, Xiuhong Xu
1
, Nuanchun Wang
3
, Xingwei Zhang
1
, Jie Ren
1
, Bingguang Xiao
4
, Jialai
Xu
3
, Weifeng Wang
1
, Chuanyi Wang
1
, Xianwei Hao
1,2
, Zhongfeng Zhang
1
1. Tobacco Research Institute of CAAS, Qingdao, 266101, P.R. China
2. Graduate School of CAAS, Beijing, 100081, P.R. China
3. Shandong Corporation of CNTC, Jinan, 250101, P.R. China
4. Yunnan Academy of Tobacco Agricultural Sciences, Yuxi, 653100, P.R. China
Corresponding author email:
zhzhf1969@163.com;
Authors
Plant Gene and Trait, 2012, Vol.3, No.6 doi: 10.5376/pgt.2012.03.0006
Received: 02 Mar., 2012
Accepted: 06 Jun., 2012
Published: 08 Jun., 2012
This article was first published in Molecular Plant Breeding (2012, 10(2): 89-94) in Chinese, and here was authorized to translate and publish the paper in
English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the
original work is properly cited..
Preferred citation for this article:
Tan et al., 2012, QTLs Related to the Easy Curing Potential Mapped in Flue-cured Tobacco, Plant Gene and Trait, Vol.3, No.6 28-33 (doi: 10.5376/pgt.
2012.03.0006)
Abstract
This genetic map drawn by Mapmaker 3.0 containing 17 linkage groups with 75 SSR markers by using a F
2
mapping
population derived from the intervarietal cross of Yunyan85 ×Dabaijin599 and 91 polymorphic markers identified by SSR molecular
marker technique. Four QTLs related to the character of easy curing potential were detected based on this genetic map and the
phenotype data by the method of composite interval mapping, which mapped on the linkage group 1, 8 and 9, respectively, with the
explainable phenotypic variation of 9.26%, 8.87%, 7.57% and 7.83%. The additive effect values of the four QTLs are all positive,
indicating these additive effects should come from one of the parent, Yunyan85.
Keywords
Flue-cured tobacco; Easy curing potential; QTL
Background
Tobacco is one of an important cash crop and well
known as an important model plant in biotechnology;
Tobacco’s genome contains 24 pairs of chromosomes.
In the past, researchers have revealed lower genetic
diversity in tobacco by using RAPD and AFLP
markers, of which has affected the progress of the
tobacco genetic map construction.
At present, the linkage maps have been reported in
tobacco as follows, Lin et al (2001) built the first
tobacco molecular marker genetic linkage map based
on the 99 individuals of the F
2
plants derived from the
cross of tobacco wild species,
Nicotiana plumbagini
-
folia
×
N. longiflora
; Nishi et al (2003) constructed a
genetic linkage map of the burley tobacco containing
10 linkage groups by using AFLP technology; Xiao et
al (2006) constructed a genetic linkage map of
flue-cured tobacco including 27 linkage groups and
169 markers consisting of molecular markers based on
ISSR and RAPD molecular markers; Julio (2006)
constructed a molecular genetic linkage map of
flue-cured tobacco consisting of 18 linkage groups
and 138 molecular markers based on 114 flue-cured
tobacco recombinant inbred lines by using ISSR,
AFLP and SSAP markers; Ma et al (2008) constructed
a genetic map containing 26 linkage groups and 112
markers by using flue-cured tobacco and burley
tobacco based on SRAP markers and ISSR markers;
Bindler et al (2011) constructed a genetic linkage map
of 2 318 SSR markers containing 24 linkage groups
and using F
2
plants derived from a cross of Hicks
Broadleaf × Red Russian. Diverse genetic maps can
be effectively applied to gene mapping of the
quantitative traits of different tobacco cultivars, posi-
tional cloning, the studies of comparative genomics
and molecular marker-assisted breeding, it would be
significant to further build the genetic linkage map of
tobacco in different cultivating types.
Easy curing potential is one of the important traits of
the tobacco, which mainly exists is reflected in
yellowing stage of the curing process, representing
the degrees of difficulty and synchronization of