Molecular Soil Biology 2015, Vol.6, No.2, 1-6
5
mesophyll cells are damaged, while most stomas are
closed and section condition, it shows that concentration
of NaHCO
3
presents significant wreck influence on
lamina structure.
Figure 3 The effect of different concentrations of NaHCO
3
stress on root structure of
S. sungkianica
×40
Note: Ep: Epidermis; Oc: Outer cortex; Cp: Cortical parenchyma; Ae: Aerenchyma; Wc: Within cortex; Px: Primary xylem; Pp:
Primary phloem
Figure 4 The effect of different concentrations of NaHCO
3
stress on leaf structure of
S. sungkianica
×40
Note: Up: Upper epidermis; Lp: Lower epidermal; Mc: Mesophyll cells; St: Stoma
In conclusion,
S. sungkianica
seeds process a certain
degree of salt-alkali resistance; when the concentration
of NaCl is below 50 mmon/L or, NaHCO
3
under 10
mmon/L, the saplings are highly adaptable and a low
concentration of NaHCO
3
could accelerate seeds
germination and seedings development; under the
suppress of a high concentration of salt or alkali, the
anatomic results show that the roots and lamina of
S.
sungkianica
all present a certain degree of resistance.
Hence the thought of using
S. sungkianica
as
reproducing tress species on salt-alkali soil is
reasonable, of which should take full advantage of
wild
S. sungkianica
resources and improves the
quality of salt-alkali soil.
2 Materials and methods
2.1 Acquisition and preservation of seeds
The
S. sungkianica
seeds were collected in late May,
2013, from
S. sungkianica
trees along the bank of
Songhuajiang river of Harbin city. Only the most
voluptuous and non-verminous were selected and
preserved in
-
80 degree for further test.
2.2 Seed germination test
NaCl concentration was set at 0, 50, 100, 150, 200,
and 250 mmol/L, and NaHCO
3
concentration was
divided into six gradients, namely, 0, 10, 20, 30, 40,
and 50 mmol/L.Put 50 seeds from each of these
concentrations on a culture dish, which is 10cm in
diameter and filled only with 0.8% of agar. All the
