Molecular Soil Biology 2015, Vol.6, No.2, 1-6
6
dishes were placed in the ZPQ
-
400 intelligent
climatron.The illumination time was set as 12 h/d,
intensity 5 grade, humidity 19
-
23 % and temperature
25
℃
. Four dishes were prepared for each concentration.
A seed was germinated when 1mm of the radicle was
observed breaking out of seed coat. Phenomena was
recorded every day. All stress treatments were
removed on the fourth day when 20 saplings were
randomly selected from each dishes for the measuring
and calculating of their length, raw weight, germination
rate (%), germination potential (%), germination
index (GI), vital index (VI) and salt-resistance.
Non-germinating seeds were placed in a container
with distilled water and subjected to germination
experiment, and germinative number was then
recorded (Guan et al., 2009).
2.3 Seeding cultivation
Based on the experiments of seed germination, the
seeding cultivation test was conducted in five groups,
the control group 0 (CK), two groups with NaCl was
50 and 100 mmon/L, and another two groups which
contend 10 and 30 mmon/L NaHCO
3
.Each plant pot
was filled with a mixture of compost and vermiculite
(1:1), the seedings were planted uniformly and covered
with a thin layer of soil, while all pots were covered
with plastic wrap to prevent water from over-evapora-
tion. Then each pot was irrigated at fixed intervals
with specific treating fluid, maintaining the soil`s
water content at 100%. Each group with six samples.
After 30 days, the seedings were picked up and
cleaned, then treated with FAA stationary liquid for 48 h
for the final preparation of paraffin section.
2.4 Anatomical observation of root and lamina
The samples were made from taproot and central
lamina, paraffin section were prepared with general
method, staining with sarranine-fast green (counter-
dying). All sections were observed with Olympus
optical microscope (BX41) and photoed by camera
(Wang et al., 2010).
2.5 Data analysis method
All data were plotted and analyzed with Excel 2003,
significance of difference and correlation analysis are
examined with SPSS 19.0. If analysis of variance was
significance, then doing multiple comparative studies
via Duncan method and equation of linear regression
(Yan et al., 2013).
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