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张秀荣等: 花生 Cu/Zn-SOD 基因(AhCSD1)克隆及品种间等位多态性分析
1080
nih.gov/Structure/cdd/wrpsb.cgi)分析蛋白保守结构
域。蛋白质基本理化性质、二级结构、跨膜结构和亚
细胞定位预测运用 ProtParam (http://www.expasy.org/)，
SOPMA (http://pbil.ibcp.fr/htm/index.php)，TMHMM
(http://www.cbs.dtu.dk/services/TMHMM/)和 softberry
(http://linux1.softberry.com/berry.phtml)完成。
作者贡献
张秀荣、万勇善、刘风珍是本研究的实验设计和实验
研究的执行人；张秀荣完成数据分析，论文初稿的写作；于
元杰、张昆参与实验设计，试验结果分析；万勇善、刘风珍
是项目的构思者和负责人，指导实验设计，数据分析，论文
写作与修改。全体作者都阅读并同意最终的文本。
致谢
本研究由国家自然科学基金项目(31201167)、国家现代
农业产业技术体系建设专项资金项目（CARS-14）、“十二五”
农村领域国家科技计划项目(2011BAD35B04)、山东省花生
良种产业化工程项目和山东省现代农业产业技术体系花生
创新团队建设项目资助。作者感谢中国农业科学院油料作物
研究所姜慧芳研究员提供花生二倍体野生种材料。
参考文献
Apel K., and Hirt H., 2004, Reactive oxygen species:
metabolism, oxidative stress, and signal transduction,
Annu. Rev. Plant Biol., 55: 373-399
http://dx.doi.org/10.1146/annurev.arplant.55.031903.141701
PMid:15377225
Chen X.P., Wang M.L., Holbrook C., Culbreath A., Liang X.Q.,
Brenneman T., and Guo B.Z., 2013, Identification and
characterization of a multigene family encoding germin-like
proteins in cultivated peanut (Arachis hypogaea L.), Plant
Molecular Biology Reporter, 29(2): 389-403
http://dx.doi.org/10.1007/s11105-010-0237-6
Dong J.Y., Wan Y.S., and Liu F.Z., 2012, Sequence analysis
ofΔ9 stearoy-ACP desaturase gene(SAD)in Peanut, Zuowu
Xuebao (Acta Agronomica Sinica), 38(7): 1167-1177 (东
金玉, 万勇善, 刘风珍, 2012, 花生 Δ9 硬脂酰-ACP 脱氢
酶基因( SAD)的序列分析, 作物学报, 38(7): 1167-1177)
Girdthai T., Jogloy S., Vorasoot N., Akkasaeng C., Wongkaew
S., Holbrook C.C., and Patanothai A., 2010, Associations
between physiological traits for drought tolerance and
aflatoxin contamination in peanut genotypes under
terminal drought, Plant Breeding, 129(6): 693-699
http://dx.doi.org/10.1111/j.1439-0523.2009.01738.x
Huang C.H., Kuo W.Y., Weiss C., and Jinn T.L., 2012, Copper
chaperone-dependent and-independent activation of three
copper-zinc superoxide dismutase homologs localized in
different cellular compartments in Arabidopsis, Plant
Physiology, 158 (2): 737-746
http://dx.doi.org/10.1104/pp.111.190223
PMid:22186608 PMCid:3271763
Jiang H.F., and Ren X.P., 2004, The effect on SOD activity and
protein content in groundnut leaves by drought stress,
Zuowu Xuebao (Acta Agronomica Sinica), 30(2): 169-174
(姜慧芳, 任小平, 2004, 干旱胁迫对花生叶片 SOD 活性
和蛋白质的影响, 作物学报, 30(2): 169-174)
Moretzsohn M.C., Gouvea E.G., Inglis P.W., Leal-Bertioli
S.C.M., Valls J.F.M., and Bertioli D.J., 2013, A study of
the relationships of cultivated peanut (Arachis hypogaea )
and its most closely related wild species using intron
sequences and microsatellite markers, Annals of Botany,
111(1): 113-126
http://dx.doi.org/10.1093/aob/mcs237
PMid:23131301
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., and
Kumar S., 2011, MEGA5: molecular evolutionary
genetics analysis using maximum likelihood, evolutionary
distance, and maximum parsimony methods, Molecular
Biology and Evolution, 28(10): 2731-2739
http://dx.doi.org/10.1093/molbev/msr121
PMid:21546353 PMCid:3203626
Wang X.C., Ma X.M., Tang F.S., and Zhang X.Y., 2002,
Changes of several protective enzyme activities of
leaves among different types of kernel of peanut after
flowering, Zhiwu Shenglixue Tongxun (Plant Physiology
Communications), 38(6): 572-574 (王小纯, 马新明, 汤丰
收, 张新友, 2002, 不同粒型品种花生开花后叶片中几种
保护酶活性的变化, 植物生理学通讯, 38(6): 572-574)
Wu B.G., 1989, Characteristics and distributions of isozymes of
superoxide dismutase in leaf cells of peanut and wheat,
Zhiwu Xuebao (Journal of Integrative Plant Biology),
31(11): 854-859 ( 武宝玕, 1989, 小麦和花生叶细胞内超
氧物歧化酶 (SOD)同工酶的特性和分布 , 植物学报,
31(11): 854-859)
Yin Y.Q., Hu J.B., and Deng M.J., 2007, Latest development of
antioxidant system and responses to stress in plant leaves,
Zhongguo Nongxue Tongbao (Chinese Agricultural
Science Bulletin), 23(1): 105-110 (尹永强, 胡建斌, 邓明
军, 2007, 植物叶片抗氧化系统及其对逆境胁迫的响应
研究进展, 中国农学通报, 23(1): 105-110)
Zhang Z.M., Dai L.X., Song W.W., Ding H., Ci D.W., Kang T.,
Ning T.Y., and Wan S.B., 2013, Effect of drought stresses
at different growth stages on peanut leaf protective
enzyme activities and osmoregulation substances content,