Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1287
-
1296
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1287
-
1296
http://mpb.5th.sophiapublisher.com
1287
研究报告
A Letter
云南省
134
个水稻新品种
DNA
指纹图谱构建和遗传多样性分析
吴毅歆
1*
,
刘春明
2*
,
毛自朝
1
,
李学进
3
,
何月秋
1,2
1.
云南农业大学农业与生物技术学院
,
昆明
, 650201
2.
云南农业大学农业生物多样性应用技术国家工程中心
,
昆明
, 650201
3.
云南省农业厅种子管理站
,
昆明
, 650031
*
同等贡献作者
通讯作者
:
ynfh2007@163.com
作者
分子植物育种
, 2012
年
,
第
10
卷
,
第
39
篇
doi: 10.5376/mpb.cn.2012.10.0039
收稿日期：
2012
年
06
月
25
日
接受日期：
2012
年
07
月
06
日
发表日期：
2012
年
08
月
08
日
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用，版权所有人允许和同意第三方无
条件的使用与传播。
引用格式
(
中文
)
：
吴毅歆等
, 2012,
云南省
134
个水稻新品种
DNA
指纹图谱构建和遗传多样性分析
,
分子植物育种
(online) Vol.10 No.39 pp.1287-1296 (doi: 10.5376/
mpb.cn.2012.10.0039)
引用格式
(
英文
)
：
Wu et al., 2012, Establishment of DNA Fingerprinting Profile and Genetic Analysis for 134 New Released Rice Varieties in Yunnn Province, Fenzi Zhiwu
Yuzhong (online) (Molecular Plant Breeding) Vol.10 No.39 pp.1287-1296 (doi: 10.5376/mpb.cn.2012.10.0039)
摘
要
利用
SSR
标记研究
134
个云南省水稻新品种的种子纯度、遗传多样性，并构建
DNA
指纹图谱。结果表明，
95.52%
品种纯度达
90%
以上，
4.48%
品种纯度低于
90%
。
12
对引物共检测出
55
个等位基因，平均每个标记
4.583 3
个，
变幅
2~7
个；平均有效等位基因总数为
4.257 5
个，变幅
1.342 0~3.771 9
个，
Shannon
多样性指数
(I)
平均值
1.133 6
，变
化幅度
0.537 8~1.502 2
；多态性信息含量
(PIC)
平均值为
0.605 5
，变动范围
0.254 8~0.734 9
。
134
个品种之间的遗传相
似性系数变异范围为
0.222 2~0.975 6
，平均遗传相似性系数为
0.592 9
，其中遗传相似性系数大于
0.500 0
的品种对占供
试品种对的
77.06%
，表明大部分供试品种之间的亲缘关系较近，遗传基础狭窄。
UPGMA
聚类分析果表明，在遗传相
似系数为
0.560 4
处，
134
个供试品种划分为
8
个类群，类群Ⅰ包括
115
个品种，占所有测试品种总数的
85.82%
，表明
供试品种的遗传背景比较单一。
关键词
水稻
;
新品种
;
简单重复序列
; DNA
指纹
;
遗传分析
Establishment of DNA Fingerprinting Profile and Genetic Analysis for 134 New
Released Rice Varieties in Yunnn Province
Wu Yixin
1*
, Liu Chunming
2*
, Mao Zichao
1
, Li Xuejin
3
, HeYueqiu
1,2
1. School of Agriculture and Biological technology, Yunnan Agricultural University (YAU), Kunming, 650201;
2. National Engineering Center for Agricultural Biodiversity, YAU, Kunming, 650201
3. Seed Management Station, Agriculture Department of Yunnan Province, Kunming, 650031
* These authors contributed equally to this paper
Corresponding author, ynfh2007@163.com;
Authors
Abstract
Simple sequence repeats (SSR) were used to detect variety purity, genetic diversity among 134 new released rice
cultivars in Yun Nan province and attempt to establish DNA fingerprinting. The results showed that, 95.52% of the tested
cultivars had 90% purity above and 4.48% of them had less 90% purity. Twelve SSR primers generated 55 polymorphic fragments.
The average number of allele per SSR locus was 4.583 3 with average from 2 to 7While the average number of effective allele per
SSR locus was 4.257 5 with average from 1.342 0 to 3.771 9. The Shannon's Information index (I) for the SSR loci varied from
0.537 8 to 1.502 2 with an average of 1.133 6, while The polymorphism information Content (PIC) for the SSR loci varied from
0.254 8 to 0.734 9 with an average of 0.605 5. Genetic similarities among the 134 new released rice cultivars ranged from 0.222 2
to 0.975 6 with an average of 0.592 9, and genetic similarities coefficient with above 0.500 0 accounted for 77.06% in the 134
new released rice cultivars. It was clear that there is closer relationship among the tested cultivars with narrow genetic ba se. The
cluster analysis showed that the tested cultivars could be classified into 4 distinct groups at 0.560 4 of the criteria of genetic
similarities coefficient, which the group
Ⅰ
contained 115 tested cultivars, accounting for 85.82 % of all cultivars. The results
indicated the narrow genetic base existed among the detected cultivars that were latest released in the Yunnan Province.
Keywords
Rice; New released cultivars; Simple sequence repeat (SSR); DNA fingerprinting; Genetic analysis