Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1511-1517
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1511-1517
http://mpb.5th.sophiapublisher.com
1513
表
1 SpoA-p
启动子序列分析
Table 1 Prediction of the promoter of SpoA-p
顺式元件
位点
核心序列
注解
Cis-element
Site
Core sequence Annotation
-
300ELEMENT
914(
-
)
TGHAAARK Upstream of the promoter from the B-hordein
gene of barley
2SSEEDPROTBANAPA
959(
-
)
CAAACAC
Important for high activity of the promoter
CANBNNAPA
959(
-
)
CNAACAC
Core of “(CA)n element” in storage protein
genes
ABRELATERD1
428(
-
)
ACGTG
Responsive todehydration
CAATBOX1
939(
-
)609(
-
)223(
-
)881(+)874(+) CAAT
Responsible for the tissue specific promoter
activity
ACGTCBOX
434(+)434 (
-
)
GACGTC
Plant bZIP protein DNA binding specificity
ANAERO1CONSENSUS
198(
-
)
AAACAAA
In promoters of 13 anaerobic genes in silico
ARR1AT
792(+)56(+)484(+)
NGATT
A response regulator
CACTFTPPCA1
423(+)690(+) 442 (
-
)482 (
-
)
YACT
A key component of mesophyll expression
module 1 in the C4 plant
CCAATBOX1
1134 (+)609 (
-
)
CCAAT
Synergistic effect of upstream sequences
CGACGOSAMY3
53(+)433(+)601 (
-
)
CGACG
Required for Amy3D expression during sugar
starvation
DPBFCOREDCDC3
956 (
-
)1107 (
-
)
ACACNNG
Transcription factors
EBOXBNNAPA
475(
-
)239(
-
)362(+)
CANNTG E-box of napA storage-protein gene
GATABOX
206(
-
)455(
-
)953 (
-
)
GATA
Required for high level and tissue specific
expression
IBOXCORE
886 (+)952 (
-
)
GATAA
Light-regulated
NTBBF1ARROLB
524 (+)
ACTTTA
Required for tissue-specific expression
OSE1ROOTNODULE
141(
-
)207(
-
)948 (
-
)
AAAGAT
Organ-specific elements of the promoters
activated in infected cells of root nodules
PYRIMIDINEBOXOSRAMY1A 546 (
-
)
CCTTTT
Partially involved in sugar repression
SREATMSD
1122(+)
TTATCC
Sugar-repressive element
TATCCAOSAMY
471 (+)
TATCCA
Alpha-amylase gene expression
WBOXHVISO1
276 (
-
)
TGACT
Sugar-responsive elements
图
3
转基因烟草的获得
Figure 3 Acquisition of transgenic tobacco plants
1.4
转基因烟草及甘薯植株的分子检测
将阴性、阳性对照及样品
DNA
进行
PCR
扩增
后，电泳检测有
15
株烟草跟阳性对照扩出同为
1 000
bp
的条带，阴性对照及另
5
株抗性植株未见条带，
由此确定
15
株烟草被成功导入了
SpoA-p
；同样有
10
株甘薯被确定为阳性植株
(
图
5)
。
1.5
转基因烟草及甘薯植株的
GUS
检测
将
GUS
染液内
37
℃浸泡过夜的阴性对照、甘薯
及烟草的根、茎、叶分用
95%
乙醇脱色至阴性对照
完全为白色，观察并照相。染色结果表明分子检测