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Basic HTML Version

分子植物育种
(
网络版
), 2012
,
10
,
1487-1492
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1487-1492
http://mpb.5th.sophiapublisher.com
1491
3
实验中所用培养基成分组成
Table 3 The medium composition used in the experiment
编号
基本成分
KT (mg/L) 2,4
-
D (mg/L) IBA (mg/L) Glucose (g/L)
Number Basic ingredients
MSB1 1/2MS
无机盐
0
0
0
15
1/2MS inorganic salts
MSB2 MS
无机盐
+B5
有机物
+MgSO4·7H2O (0.12 g/L)
0.1
0.1
0
30
MS inorganic salts+B5 organic matter+MgSO4·7H2O (0.12 g/L)
MSB3 MS
无机盐
+B5
有机物
0.1
0.05
0
30
MS inorganic salts+B5 organic matter
MSB4 MS
无机盐
(
去除
NH4NO3, KNO3
加倍
)+B5
有机物
+Gln
(1.0 g/L) +Asn (0.5 g/L)
0.15
0
0.5
30
MS inorganic salts (removal of NH4NO3, doubling of
KNO3)+B5 organic matter+Gln (1.0 g/L)+Asn (0.5 g/L)
MSB5 1/2MS
无机盐
(
去除
NH4NO3)+B5
有机物
+Gln (1.0 g/L)+Asn
(0.5 g/L)
0
0
0
15
1/2MS inorganic salts (removal of NH4NO3)+B5 organic
matter+Gln (1.0 g/L)+Asn (0.5 g/L)
每天光培养
14 h
,暗培养
10 h
作者贡献
李雪林、王翠玲和孟超敏是本研究的实验设计和实验
研究的执行人;李雪林完成数据分析;王翠玲参与实验设计,
试验结果分析;孟超敏是项目的构思者和负责人,指导实验
设计,数据分析,论文写作与修改;全体作者都阅读并同意
最终的文本。
致谢
本研究由河南科技大学博士启动基金
(09001463)
和河
南科技大学青年科学基金
(2011QN038)
共同资助。感谢河南
科技大学农学院的熊晨琳、赵倩同学在本实验过程中提供
的帮助。
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