Page 20 - 2012no62

分子植物育种(网络版), 2012 年, 第 10 卷, 第 1458-1463 页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1458-1463
http://mpb.5th.sophiapublisher.com
1462
3.6 转基因小麦的纹枯病抗性鉴定
采用病麦粒接种法鉴定转基因小麦的纹枯病
抗性。拔节期时将带有禾谷丝核菌 R. cerealis R0301
菌丝的病麦粒均匀播于转基因小麦植株基部，弥雾
保湿 7 d。小麦乳熟期时调查纹枯病发病情况，每个
转基因株系随机调查 15 个发病茎秆，重复 2 次，病
级调查按 1~5 级标准，1 级：叶鞘有典型病斑，病菌
不侵入茎杆；2 级：病菌侵入茎秆，但病斑宽度不超
过茎秆周长的 1/2；3 级：病斑宽度在 1/2~3/4 茎秆周
长之间；4 级 ：病斑宽度占茎秆周长的 3/4 以上；5
级：枯孕穗或枯白穗。分别计算各转基因株系平均
病级和平均病情指数。平均病情指数={(1×χ1+2×χ2+3-
×χ3+4×χ4+5×χ5)/[(χ1+χ2+χ3+χ4+χ5)×5]}×100，式中：χ1,
χ2,…，χ5分别代表 1,2,……5 级的茎秆数。采用 SAS 9.0
软件进行方差分析。
作者贡献
周淼平、杨学明、张鹏和余桂红是本研究的实验设计和
实验研究的执行人；周淼平是项目的构思者及负责人，完成
数据分析和论文初稿的写作；姚金保和马鸿翔指导实验设计
和论文修改。全体作者都阅读并同意最终的文本。
致谢
本研究由国家转基因生物新品种培育重大专项(2011-
ZX08002001)、江苏省产学研联合创新基金(BY2012208)、江
苏省科技支撑计划项目(BE2011306)、国家科技支撑项目
(2011BAD35B03)和江苏省农业科技自主创新资金(CX11-
1025)共同资助。感谢江苏省里下河农科所小麦室提供扬麦
12号小麦种子、江苏省农科院植保所陈怀谷研究员提供禾谷
丝核菌R0301。
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