Page 24 - 2012no61

分子植物育种(网络版), 2012 年, 第 10 卷, 第 1447- 1457 页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1447- 1457
http://mpb.5th.sophiapublisher.com
1456
表 12 不同培养基组分(王满, 2011)
Table 12 Components in the different mediums (Wang, 2011)
培养基编号 培养基类型 培养基组分
Number of medium Type of medium Components of medium
A 诱导培养基 M8+2 mg/L 2,4- D+1 mg/LABA+8 g/LAgar+30 g/L sugar
B Induction medium M8+2 mg/L 2,4- D+0.5 mg/L 6- BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
C M8+2 mg/L 2,4- D+1 mg/L 6-BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
D M8+2 mg/L 2,4- D+1.5 mg/L 6- BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
M1 继代培养基 M8+1 mg/L 2,4- D+2 mg/L 6-BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
Y1 Subculture medium M8+1 mg/L 2,4- D+1 mg/L 6-BA+0.3 mg/L KT+8 g/LAgar+30 g/L sugar
M4 N6+1 mg/L 2,4 -D+1 mg/L 6- BA+8 g/LAgar+30 g/L sugar
M5 N6+2 mg/L 2,4 -D+1 mg/L 6- BA+8 g/LAgar+30 g/L sugar
M16 M8+0.5 mg/L 2,4-D+0.2 mg/L 6- BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
M8 M8+1 mg/L 2,4- D+0.5 mg/L 6- BA+0.5 mg/LABA+8 g/LAgar+30 g/L sugar
M2 M8+2 mg/L 2,4- D+1 mg/L 6-BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
M3 M8+2 mg/L 2,4- D+1.5 mg/L 6- BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
M6 M8+1 mg/L 2,4- D+0.5 mg/L 6- BA+0.5 mg/LABA+8 g/LAgar+30 g/L sugar
M7 M8+2 mg/L 2,4- D+1 mg/L 6-BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
M8 M8+1 mg/L 2,4- D+2 mg/L 6-BA+1 mg/LABA+8 g/LAgar+30 g/L sugar
致谢
本研究由国家自然科学基金项目(30871459) 和江苏省
自主创新课题[CX(11)1020]共同资助。
参考文献
Bandyopadhyay A., Datta K., Zhang J., Yang W., Raychaudhuri
S., Miyao M., and Datta S.K., 2007, Enhanced photo-
synthesis rate in genetically engineered indica rice
expressing pepc gene cloned from maize, Plant Sci.,
172(6): 1204-1209 http://dx.doi.org/10.1016/j.plantsci.
2007.02.016
Chen P.B., and Li X., 2012, Promotion of photosynthesis of
transgenic rice plant with overexpressing C4 pepc from
maize under low concentration NO, Zhiwu Yanjiu
(Bulletin of Botanical Research), 32(4): 402-409 (陈平波,
李霞, 2012, 低浓度NO对高表达转玉米C4型pepc水稻光
合的促进, 植物研究, 32(4): 402-409)
Chen Z., Chen J.H., Bian L.M., Li T.T., Wu C., and Shi J.S.,
2007, Establishment of embryogenic callus suspension
culture system in Liriodendron hybrids, Fenzi Zhiwu
Yuzhong (Molecular Plant Breeding), 5(1): 137-140 (陈志,
陈金慧, 边黎明, 李婷婷 , 吴淳, 施季森, 2007, 杂交鹅
掌楸胚性细胞悬浮系的建立, 分子植物育种 , 5(1):
137-140)
Fang W.J., Han L.B., and Zeng H.M., 2005, Research advances
in factors affecting establishment of plant cell suspension
culture, Shengwu Jishu Tongbao (Biotechnology Infor-
mation), (5): 11-15 ( 方文娟, 韩烈保, 曾会明, 2005, 植
物细胞悬浮培养影响因子研究 , 生物技术通报 , (5):
11-15)
Hu Y.M., Guo Y.G., Jiang X.W., Yu Q.D., and Cui P., 2010,
Effect of sucrose and natural organic affixtures on
flavonoids biosynthesis of Ginkgo Callus, Xinan Nongye
Xuebao (Southwest China Journal of Agricultural
Sciences), 23(3): 690-693 (胡燕梅, 郭云贵, 蒋细旺, 余
群娣, 崔鹏, 2010, 蔗糖及天然有机物对银杏愈伤组织合
成黄酮的影响, 西南农业学报, 23(3): 690-693)
Ku M.S., Agarie S., Nomura M., Fukayama H., Tsuchida H.,
Ono K., Hirose S., Toki S., Miyao M., and Matsuoka M.,
1999, High-level expression of maize phosphoenol-
pyruvate carboxylase in transgenic rice plants., Nat.
Biotechno1., l7: 76-80
Li X., Wu S., Jiao D.M., Wang S.H., Li C.Q., and Ku M.S.B.,
2001, Selection and breeding of the pepc transgenic rice,
Jiangsu Nongye Xuebao (Jiangsu Journal of Agricultural
Sciences), 17(3): 143-147 (李霞, 吴爽, 焦德茂, 王守海,
李成荃, 古森本, 2001, 转pepc基因水稻的选育, 江苏
农业学报, 17(3): 143-147)
Li X., Jiao D.M., and Dai C.C., 2005a, The Response to
photooxidation in leaves of PEPC transgenic rice plant
(Oryza sativa L.), Zuowu Xuebao (Acta Agronomica
Sinica), 31(4): 408-413 (李霞, 焦德茂, 戴传超, 2005a,