Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1447
-
1457
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1447
-
1457
http://mpb.5th.sophiapublisher.com
1449
表
1
不同
6
-
BA
浓度对水稻发芽率与出愈率的影响
Table 1 Effects of different concentrations of 6
-
BA on the rates of buding and callus induction of rice
水稻品种
6
-
BA
接种数量
出芽率
(%)
14 d
后出愈率
(%)
Rice variety
(mg/L)
Inoculationnumber
Buding rate (%)
Callus induction rate after 14 d of inoculation (%)
PC
0
50
96.10±1.10
7.20±0.30
0.5
50
98.10±1.00
0.00±0.00
1
50
96.20±1.30
0.00±0.00
1.5
50
100.00±0.00
3.10±0.10
WT
0
50
100.00±0.00
10.20±0.40
0.5
50
100.00±0.00
18.10±0.60
1
50
100.00±0.00
10.20±0.50
1.5
50
100.00±0.00
8.10±0.30
注
:
表
1
中使用的培养基是以基本培养基
M8
的大量元素
+
微量元素
+
有机成份为基本培养基
,
再添加
2 mg/L 2,4
-
D, 30 g/L
蔗
糖和
8 g/L
琼脂
, pH 5.8,
愈伤组织诱导温度
(22
±
2)
℃
Note: Medium used in figure 1 was supplemented with 2,4
-
D 2 mg/L, 30 g/L sugar and 8 g/L agar based on basic medium including
the large elements of M8 medium plus trace elements and organic ingredients, pH 5.8, induction temperature at (22
±
2)
℃
表
2 ABA
和
6
-
BA
对供试水稻发芽率和出愈率的影响
Table 2 Effects of different concentrations of 6
-
BA and ABA on the rates of buding and callus induction of rice
水稻品种
6
-
BA
ABA
接种数量
7 d
发芽率
(%)
出愈率
(%)
Rice variety (mg/L) (mg/L) Inoculation number
Buding rate after 7 d (%)
Callus induction rate after 7 d (%)
PC
1.5
1
50±2.00
100.00±0.00
3.50±0.01
1.5
0
90±3.00
98.10±0.50
0.00±0.00
0
0
80±3.00
99.10±0.10
0.00±0.00
WT
1.5
1
50±2.00
100.00±0.00
8.20±0.04
1.5
0
60±2.00
92.10±0.20
0.00±0.00
0
0
100±3.00
95.10±0.10
3.10±0.02
注
:
表
2
中使用的培养基是以基本培养基
M8
的大量元素
+
微量元素
+
有机成份为基本培养基
,
再添加
2 mg/L 2,4
-
D, 30 g/L
蔗
糖和
8 g/L
琼脂
, pH 5.8,
愈伤组织诱导温度
(22
±
2)
℃
Note: Medium used in figure 2 was supplemented with 2,4
-
D 2 mg/L,30 g/L sugar and 8 g/L agar based on basic medium including
the large elements of M8 medium plus trace elements and organic ingredients, pH 5.8, induction temperature at (22
±
2)
℃
表
3
单独添加不同
2,4
-
D
浓度对供试水稻诱导愈伤组织的影响
Table 3 Effects of different concentrations of 2,4
-
D on the rate of callus induction rates of rice
水稻品种
2,4
-
D
接种数量
7 d
出愈率
(%)
14 d
出愈率
(%)
21 d
出愈率
(%)
Rice variety (mg/L)
Inoculation number
Callus induction
Callus induction
Callus induction
rate for 7 d (%)
rate for 14 d (%)
rate for 21 d (%)
PC
1
50±2.00
0.00±0.00
0.00±0.00
5.10±0.20
2
50±2.00
0.00±0.00
0.00±0.00
5.20±0.10
3
50±3.00
0.00±0.00
6.20±0.30
10.10±0.20
4
48±2.00
0.00±0.00
10.00±0.20
13.50±0.40
5
64±3.00
0.00±0.00
9.00±0.30
10.20±0.20
WT
1
50±2.00
0.00±0.00
6.10±0.20
13.20±0.10
2
66±3.00
0.00±0.00
6.20±0.30
13.30±0.20
3
40±2.00
13.30±0.20
20.50±0.20
23.40±0.30
4
40±1.00
10.20±0.10
20.40±0.10
17.10±0.20
5
48±2.00
0.00±0.00
27.50±0.90
40.40±1.10
注
:
表
3
中使用的培养基是以基本培养基
N6
的大量元素
+
微量元素
+
有机成份为基本培养基
,
再添加
30 g/L
蔗糖和
8 g/L
琼脂
,
pH 5.8,
愈伤组织诱导温度
(22±2)
℃
Note: Medium used in figure 3 was supplemented with 30 g/L sugar and 8 g/L agar based on basic medium including the large
elements of N6 medium plus trace elements and organic ingredients, pH 5.8, induction temperature at (22±2)
℃