Basic HTML Version
分子植物育种
(
网络版
), 2012
年
,
第
10
卷
,
第
1092
-
1096
页
Fenzi Zhiwu Yuzhong (Online), 2012, Vol.10, 1092
-
1096
http://mpb.5th.sophiapublisher.com
1096
工作紧系衔接的情况下，
dbMarker
的设计目标就定
位于弥补上述缺口；其次，该软件的体积非常小巧，
源文件只有
30
多
K
，而且
Google App Engine
平台
为软件的发布和使用提供了极大的便利性，更不需
要用户进行硬件方面的投入，大大降低了应用难度
和技术风险。目前，该软件已经获得国家版权局授
予的软件著作权保护登记
(2010SR059772)
，并在
Google
代码网站上设立了项目托管，访问地址为：
http://code.google.com/p/dbmarker/
。用户可以通过以
上地址了解技术信息，下载软件包以及源代码。
作者贡献
任民、盖红梅及蒋彩虹是本研究的实验设计和实验研究
的执行人；任民负责系统架构和软件开发；盖红梅和蒋彩虹
负责系统测试，任民、盖红梅完成了论文初稿的写作；王志
德是项目的构思者及负责人，指导实验设计，数据分析，论
文写作与修改。全体作者都阅读并同意最终的文本。
致谢
本研究由中国农业科学院烟草研究所所长基金
“
应用选
择牵连效应发掘烟草基因组保守位点
”
、中央级公益性科研
院所基本科研业务费专项
(0032010038)
和青岛市科技计划
基础研究项目
(10-3-4-14-1-jch)
共同资助。
参考文献
Bombarely A., Menda N., Tecle I.Y., Buels R.M., Strickler S.,
Fischer-York T., Pujar A., Leto J., Gosselin J., and Mueller
L.A., 2011, The sol genomics network (solgenomics.net):
growing tomatoes using perl, Nucleic Acids Res.,
39(Database issue): D1149- D1155
Dudley J.T., and Butte A.J., 2010, In silico research in the era
of cloud computing, Nat. Biotechnol., 28(11): 1181-1185
http://dx.doi.org/10.1038/nbt1110-1181 PMid:21057489
Galperin M.Y., and Fernández-Suárez X.M., 2011, The 2012
nucleic acids research database issue and the online
molecular biology database collection, Nucleic Acids Res.,
40(Database issue): D1-D8
Huala E., Dickerman A.W., Garcia-Hernandez M., Weems D.,
Reiser L., LaFond F., Hanley D., Kiphart D., Zhuang JM.,
Huang W., Mueller L.A., Bhattacharyya D., Bhaya D.,
Sobral B.W., Beavis W., Meinke D.W., Town C.D.,
Somerville C., and Rhee S.Y., 2001, The arabidopsis
information resource (TAIR): a comprehensive database
and web-based information retrieval, analysis, and
visualization system for a model plant, Nucleic Acids
Res., 29(1): 102-105 http://dx.doi.org/10.1093/nar/29.1.102
PMid:11125061 PMCid:29827
Kim C.K., Kim J.S., Lee G.S., Park B.S., and Hahn J.H., 2008,
PlantGM: a database for genetic markers in rice (
Oryza
sativa
) and Chinese cabbage (
Brassica rapa
), Bioinformation,
3(2): 61-62 http://dx.doi.org/10.6026/97320630003061
PMid:19238232 PMCid:2637951
Kim C.K., Yoon U.H., Lee G.S., Lee H.K., Kim Y.H., and Hahn
J.H., 2009, Rice genetic marker database: An identification of
single nucleotide polymorphism (SNP) and quantitative
trait loci (QTL) markers, African Journal of Biotechnology,
8(13): 2963-2967
Langmead B., Schatz M.C., Lin J., Pop M., and Salzberg S.L.,
2009, Searching for SNPs with cloud computing, Genome
Biology, 10(11): R134 http://dx.doi.org/10.1186/gb-2009
-10-11-r134 PMid:19930550 PMCid:3091327
Rodríguez-Suárez C., Giménez M.J., Gutiérrez N., Avila C.M.,
Machado A., Huttner E., Ramírez M.C., Martín A.C.,
Castillo A., Kilian A., Martín A., and Atienza S.G., 2011,
Development of wild barley (Hordeum chilense)-derived
DArT markers and their use into genetic and physical
mapping, Theor. Appl. Genet., 124(4): 713-722 http://dx.doi.org/
10.1007/s00122-011-1741-2 PMid:22048641
Sayers E.W., Barrett T., Benson D.A., Bolton E., Bryant S.H.,
Canese K., Chetvernin V., Church D.M., Dicuccio M.,
Federhen S., Feolo M., Fingerman I.M., Geer L.Y.,
Helmberg W., Kapustin Y., Krasnov S., Landsman D.,
Lipman D.J., Lu Z., Madden T.L., Madej T., Maglott D.R.,
Marchler-Bauer A., Miller V., Karsch-Mizrachi I., Ostell J.,
Panchenko A., Phan L., Pruitt K.D., Schuler G.D.,
Sequeira E., Sherry S.T., Shumway M., Sirotkin K., Slotta
D., Souvorov A., Starchenko G., Tatusova T.A., Wagner L.,
Wang Y., Wilbur W.J., Yaschenko E., and Ye J., 2011,
Database resources of the national center for
biotechnology information, Nucleic Acids Res.,
40(Database issue): D13-D25
Sherry S.T., Ward M.H., Kholodov M., Baker J., Phan L.,
Smigielski E.M., and Sirotkin K., 2001, dbSNP: the NCBI
database of genetic variation, Nucleic Acids Res., 29(1):
308-311 http://dx.doi.org/10.1093/nar/29.1.308 PMid:11125122
PMCid:29783
Tautz D., 1989, Hypervariability of simple sequences as a
general source for polymorphic DNA markers, Nucleic
Acids Res., 17 (16): 6463-6471 http://dx.doi.org/10.1093/
nar/17.16.6463 PMid:2780284 PMCid:318341
Williams J.G.K., Kubelik A.R., Livak K.J., Rafalskil J.A., and
Tingeyl S.V., 1990, DNA polymorphisms amplified by
arbitrary primers are useful as genetic markers, Nucleic
Acids Res., 18(22): 6531-6535 http://dx.doi.org/10.1093/nar/
18.22.6531 PMid:1979162 PMCid:332606
Yu H.H., Xie W.B., Wang J., Xing Y.Z., Xu C.G., Li X.H., Xiao
J.H., and Zhang Q.F., 2011, Gains in QTL detection using
an ultra-high density SNP map based on population
sequencing relative to Traditional RFLP/SSR Markers,
PLoS One, 6(3): e17595
http://dx.doi.org/10.1371/annotation/f2eb75fb-ae22-4a57-
b828-1506aa506c6d
http://dx.doi.org/10.1371/journal.pone.0017595
PMid:21390234 PMCid:3048400
Zietkiewicz E., Rafalski A., and Labuda D., 1994, Genome
fingerprinting by simple sequence repeat (SSR)-anchored
polymerase chain reaction amplification, Genomics, 20 (2):
176-183 http://dx.doi.org/10.1006/geno.1994.1151 PMid:8020964