Page 24 - 2011no98

孙小艳等, 2011, 杂种马褂木 EST-SSR 引物开发及多态性分析, 分子植物育种(online) Vol.9 No.98 pp.1706-1712 (doi: 10.5376/mpb.cn. 2011.09.0098)
1711
-0.7/cycle)，72℃ 30 s，16 cycles；在进入94 ℃ 15 s，
50℃ 15 s，72℃ 30 s，15 cycles；72℃延伸1 h；12℃
保存。
3.5 PCR产物银染检测
SSR标记的PCR 产物在8% 的聚丙烯酰胺凝胶
上电泳，120 V，600 mA，1.5 h，银染检测。
作者贡献
孙小艳、李彦强、江聪是本研究的实验设计和实验研究
的执行人；孙小艳负责完成数据检索分析、实验结果统计分
析，论文初稿的写作；李彦强、江聪参与实验设计，试验结
果分析；徐立安是指导实验设计及数据分析；余发新是项目
策划者及负责人，指导论文写作与修改。全体作者都阅读并
同意最终的文本。
致谢
本研究由江西省科学技术厅自然科学基金资助项目
(2010GZN0067)和江西省科学技术厅科技支撑计划资助项目
(2009BNA06100)。作者感谢南京林业大学徐立安教授和江
聪、王宗伟博士在本实验过程中的技术支持和有益的建议。
感谢感谢两位匿名的同行评审人的评审建议和修改建议。
参考文献
An Z.W., Zhao Y.H., Chen H., Li W.G., and Huang H.S., 2009,
Development and application of EST-SSR markers in
Hevea brasiliensis Muell. Arg., Yichuan (Hereditas),
31(3): 311-319 (安泽伟, 赵彦宏, 程汉, 李维国, 黄华
孙, 2009, 橡胶树EST-SSR 标记的开发与应用 , 遗传 ,
31(3): 311-319)
Bérubé Y., Zhuang J., Rungis D., Ralph S., Bohlmann J., and
Ritland K., 2006, Characterization of EST-SSRs in
loblolly pine and spruce, Tree Genetics &Genomes, 3(3):
251-259
Chen Y.H., Li L.Z., Wei X.Y., Li S.S., Lei T.D., Hu H.Z., Wang
H.G., and Zhang X.S., 2005, Development of EST-SSR
markers, chromosome location and genetic mapping in
wheat, Kexue Tongbao (Chinese Science Bulletin), 50
(20): 2208-2216 (陈海梅, 李林志, 卫宪云, 李斯深, 雷
天东, 胡海洲, 王洪刚, 张宪省, 2005, 小麦EST-SSR标
记的开发, 染色体定位和遗传作图, 科学通报, 50(20):
2208-2216)
Doyle J.J., and Doyle J.L., 1990, Isolation of plant DNA from
fresh tissue, Focus, 12: 13-15
Gao L.F, Tang J.F, Li H.W., and Jia J.Z., 2003, Analysis of
microsatellites in major crops assessed by computational
and experimental approaches, Mol. Breed., 12(3): 245-
261
Huang L.B., Shi S.Z., and Zhu L.L., 2008, Primary study on
planting experiment of the clone selection of
Liriodendron chinense ×tulipifera , Jiangsu Linye Keji
(Jiangsu Forestry Science & Technology), 35(6): 1-4 (黄
利斌, 施士争, 祝良林, 2008, 杂交马褂木无性系造林
试验初报, 江苏林业科技, 35(6): 1-4)
Jung S., Abbott A., Jesudurai C., Tomkins J., and Main D.,
Frequency, type, distribution and annotation of simple
sequence repeats in Rosaceae ESTs, Funct. Integr.
Genom., 2005, 5(3): 136-143
Kantety R.V., La Rota M., Matthews D.E., and Sorrells M.E.,
2002, Data mining for simple sequence repeats in
expressed sequence tags from barley, maize, rice,
sorghum and wheat, Plant Mol. Bio., 48(5-6): 501-510
Portis E., Nagy L., Sasvári Z., Stágel A., Barchi L., and Lanteri
S., 2007, The design of Capsicum spp. SSR assays via
analysis of in silico DAN sequence and their potential
utility for genetic mapping, Plant Science, 172(3): 640-
648
Rota L.R, Kantety R.V, Yu J.K., and Sorrells M.E, 2005,
Nonrandom distribution and frequencies of genomic and
EST-derived microsatellite markers in rice, wheat, and
barly, BioMed Central, 6(1): 23
Rungis D., Berube Y., Zhang J., Ralph S., Ritland C.E., Ellis
B.E., Douglas C., Bohlmann J., and Ritland K., 2004,
Robust simple sequence repeat markers for spruce (Picea
spp.) from expressed sequence tags, Theor. Appl. Genet.,
109(6): 1283-1294
Scott K.D., Eggler P., Seaton G., Rossetto M., Ablett E.M., Lee
L.S. and Henry R.J., 2000, Analysis of SSRs derived
from grape ESTs, Theor. Appl. Genet., 100(5): 723-726
Sun Y.G., and Li H.G., 2007, The Paternity Analysis for
Open-pollination Progenies of Liriodendron L. Using
SSR Markers, Zhiwuxue Tongbao (Chinese Bulletin of
Botany), 24(5): 590-596 (孙亚光, 李火根, 2007, 利用
SSR分子标记对鹅掌楸自由授粉子代的父本分析 , 植
物学通报, 24(5): 590-596)
Varshney R.K., Thomas T., Nils S., Peter L., and Andreas G.,
2002, In silico analysis on frequency and distribution of
microsatellites in ESTs of some cereal speci, Cellular &
Molecular Biologyletters, 7(2A): 537-546
Wang Z.R., 2005, Cross breeding and utilize of Liriodendron
tulipifera, Chinese forestry Publish Company, Beijing,
China, pp.70-73 (王章荣, 2005, 鹅掌楸属树种杂交育