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分子植物育种
(
网络版
), 2011
年
,
第
9
卷
,
第
1700
-
1705
页
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1700
-
1705
http://mpb.
5th
.sophiapublisher.com
1700
研究报告
A Letter
火鹤胚状体诱导与发生过程中愈伤组织细胞学观察
王爱香
,
张克中
,
贾月慧
,
彭娇
北京农学院园林学院
,
北京
, 102206
通讯作者
:
zkzzxd@vip.sina.com
作者
分子植物育种
, 2011
年
,
第
9
卷
,
第
97
篇
doi: 10.5376/mpb.cn.2011.09.0097
收稿日期：
2011
年
07
月
21
日
接受日期：
2011
年
08
月
08
日
发表日期：
2011
年
08
月
17
日
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式
(
中文
)
：
王爱香等
, 2011,
火鹤胚状体诱导与发生过程中愈伤组织细胞学观察
,
分子植物育种
(online) Vol.9 No.97 pp.1700-1705 (doi: 10.5376/mpb.cn.
2011.09.0097)
引用格式
(
英文
)
：
Wang et al., 2011, Histocytology Observation on Anthurium andraeanum Callus during Embryoid Induction Stage and Development Stage, Fenzi Zhiwu
Yuzhong (online) (Molecular Plant Breeding) Vol.9 No.97 pp. 1700-1705 (doi: 10.5376/mpb.cn.2011.09.0097)
摘
要
本研究以火鹤“阿拉巴玛”幼嫩叶片为材料，采用石蜡切片技术对其胚状体诱导和发生过程中愈伤组织进行了
细胞学观察。结果表明：叶片在
1/2MS+0.6 mg/L 2,4
-
D+0.5~1.0 mg/L 6
-
BA
以及
1/2MS+0.4 mg/L 2,4
-
D+1.0 mg/L 6
-
BA
培
养基中获得了较高的愈伤诱导率；该愈伤组织表面光滑、紧实，显微观察可见有许多愈伤组织结节和芽原基的分布，未
观察到胚状体发生；上述愈伤组织转至
1/2MS+1.0 mg/L 6
-
BA+0.1 mg/L KT+0.5 mg/L NAA
的培养基中进行继代培养，愈
伤组织表面形成不定芽，并在原愈伤组织切块边缘形成新愈伤组织。新愈伤组织黄绿色、表面不光滑、质地疏松。显微
观察显示：在新愈伤组织表面及内部均有胚状体的发生，一些多细胞原胚与周围愈伤组织产生明显的界限，在一些多细
胞原胚表面又形成新的原胚分化中心，愈伤组织中的体细胞胚发生呈现不同步性和多样性。
关键词
火鹤
;
胚状体诱导
;
胚状体发生
Histocytology Observation on
Anthurium andraeanum Callus
during Embryoid
Induction Stage and Development Stage
Wang Aixiang , Zhang Kezhong , Jia Yuehui , Peng Jiao
College of Landscape, Beijing Agricultural college, Beijing, 102206, P.R. China
Corresponding author, zkzzxd@vip.sina.com;
Authors
Abstract
Histocytology study was done by paraffin section technique on the callus which was generated during the embryoid
induction stage and the development stage via young leaf explants of
Anthurium andraeanum
‘alabama’. The higher callus induction
rate was obtained in the mediums of 1/2MS+0.6 mg/L 2,4
-
D+0.5~1.0 mg/L 6
-
BA and 1/2MS+0.4 mg/L 2,4
-
D+1.0 mg/L 6
-
BA. The
induced callus surface appeared to be smooth and compact. Some callus nodules, adventitious bud primordiums were observed in it,
but none embryoid was observed. The induced callus was transferred into the subculture medium of 1/2MS+1.0 mg/L 6
-
BA+0.1 mg/L
KT+0.5 mg/L NAA. After 20~30 subculture days, lots of adventitious buds were initiated from the callus, ‘new callus’ was regenerated
from the former callus section edge. The new callus surface was showed to be yellow-green, rough and loose. By means of microscope
technique, embryoids were observed to be generated from both the surface and the internal cell of the ‘new callus’, obvious boundary
was exhibited between the multi-cells pro-embryo and its surrounding tissues, new pro-embryo differentiation centers were formed in
the surface of some multi-cells pro-embryos. There were desynchronization and diversity phenomenon during somatic embryogenesis in
the new callus.
Keywords
Anthurium andraeanum
; Embryoid induction; Embryoid development
研究背景
火鹤
(
Anthurium andraeanum
)
组培苗生产可通
过不定芽发生途径及胚状体发生途径两种方式
(Beyramizade et al., 2008)
。相对于前者，胚状体发
生途径有如下优点：体细胞胚再生的植株多数情况
起源于单个胚性细胞，不会出现遗传嵌合、遗传突