Basic HTML Version
分子植物育种
(
网络版
), 2011
年
,
第
9
卷
,
第
1626
-
1631
页
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1626
-
1631
http://mpb.
5th
.sophiapublisher.com
1626
研究报告
A Letter
黄瓜抗黑斑病相关
AFLP
标记筛选及
SCAR
转化
王惠哲
,
李淑菊
,
杨瑞环
,
管炜
天津科润黄瓜研究所
,
天津
, 300192
通讯作者
: wanghuizhe@126.com;
作者
分子植物育种
, 2011
年
,
第
9
卷
,
第
87
篇
doi: 10.5376/mpb.cn.2011.09.0087
收稿日期：
2010
年
06
月
07
日
接受日期：
2011
年
06
月
27
日
发表日期：
2011
年
06
月
30
日
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式：
王惠哲等
, 2011,
黄瓜抗黑斑病相关
AFLP
标记筛选及
SCAR
转化
,
分子植物育种
Vol.9 No.87 (doi: 10.5376/mpb.cn.2011.09.0087)
摘
要
为筛选与黄瓜抗黑斑病相关基因连锁的分子标记，探讨黄瓜抗病材料鉴定和选择的分子方法。以黄瓜感黑斑病母本
L63
和抗黑斑病父本
L9
及其
F
2
代分离群体为试材，人工接种抗病性鉴定结果表明，一对隐性基因控制黄瓜黑斑病的抗病性，
感病性为不完全显性。通过
BSA
法和
AFLP
分子标记建立了黄瓜对黑斑病的抗病组和感病组，应用
1 024
对引物组合
Eco
R
Ⅰ-
NN/
Mse
Ⅰ-
NNN
对抗、感组进行筛选，筛选到了
1
个与黄瓜抗、感黑斑病相关基因连锁的共显性标记
E-AT/M-TCT
。测
序结果表明，片段长度分别为
246 bp
和
251 bp
，为发生插入
/
缺失突变的同源序列；经组外其它
F
2
单株分析，在抗病个体中
扩增出了
246 bp
的特异片段，而感病个体中扩增出了
251 bp
的特异片段，中间类型单株则同时扩增出该两个特异片段，该
特异标记与黄瓜黑斑病抗性相关基因紧密连锁，遗传距离为
4.96 cM
；根据测序结果，将其成功转化为
1
对
SCAR
标记。
关键词
黄瓜
;
黑斑病
;
分子标记
; AFLP; SCAR
Screening of the AFLP Markers Associated to the Resistance Locus of
Cucumber alternaria leaf spot and Its Conversion to SCAR Marker
Wang Huizhe , Li Shuju , Yang Ruihuan , Guan Wei
Tianjin Cucumber Research Institute, Tianjin, 300192, P.R. China
Corresponding author, wanghuizhe@126.com;
Authors
Abstract
The study screened the molecular marker linked to the cucumber alternaria leaf spot resistant gene, and discussed the
molecular method for identification and selection of resistant materials. With a F
2
population derived from F
1
of L63 (susceptible
female) and L9 (resistant male) was used as materials, their resistance to alternaria leaf spot of cucumber were identified through
seedling inoculation. The results indicated that the resistance to alternaria leaf spot was controlled by a pair of recessive gene, and the
susceptible was in the mode of incomplete dominant inheritance. The polymorphism between resistant and susceptible bulk of
cucumber alternaria leaf spot were studied using BSA method and AFLP technology. 1 024 AFLP markers were screened, and a
co-dominant marker E-AT/M-TCT was obtained. Sequencing of the polymorphic fragment indicated that their lengths were 246 bp
and 251 bp, respectively, and they were orthologous sequences with an insertion/deletion of five nucleotides. The resistant and
susceptible F
2
plants possessed the 246 bp and the 251 bp fragments, the mid-plants possessed both two fragments. The marker was
closely linked to the cucumber alternaria leaf spot resistance-related gene, and the genetic distance between the marker and the gene
was 4.96 cM. According to its sequencing result, the AFLP marker was further converted into SCAR marker.
Keywords
Cucumber; Alternaria leaf spot; Molecular marker; AFLP; SCAR
研究背景
黄瓜黑斑病
[
Alternaria cucumerina
(Ell et Ev.)
Elliott.]
是由瓜链格孢菌引起的真菌性病害
(
吕佩珂
等
, 1992)
。该病近几年又卷土重来，在各地普遍发
生，成为黄瓜生产上的重要病害。该病主要危害叶
片，一般先从黄瓜的中、下部叶片开始发生，而后
逐渐向上蔓延，大约在
4
月中旬至
5
月上、中旬发生，
发病率高达
60%
以上，减产
10%~20% (
梁金兰
,
1992)
。而生产上所采用的很多材料、品种多不抗黑
斑病，因此抗黑斑病又成为培育黄瓜新品种的一个
重要指标。常规的抗病材料筛选及抗病品种选育方
法存在费时、费力、效率低、病菌扩散等问题，分