Basic HTML Version
分子植物育种
(
网络版
), 2011
年
,
第
9
卷
,
第
1570
-
1578
页
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1570
-
1578
http://mpb.
5th
.sophiapublisher.com
1570
研究报告
A Letter
基于
ITS
和
cpDNA
序列的中国野生葡萄分子系统进化
张永辉
,
樊秀彩
,
张颖
,
孙海生
,
姜建福
,
刘崇怀
中国农业科学院郑州果树研究所
,
郑州
, 450009
通讯作者
: iuchonghuai@caas.net.cn;
作者
分子植物育种
, 2011
年
,
第
9
卷
,
第
78
篇
doi: 10.5376/mpb.cn.2011.09.0078
收稿日期：
2011
年
05
月
16
日
接受日期：
2011
年
06
月
13
日
发表日期：
2011
年
06
月
22
日
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式：
张永辉等
, 2011,
基于
ITS
和
cpDNA
序列的中国野生葡萄分子系统进化
,
分子植物育种
Vol.9 No.78 (doi: 10.5376/mpb.cn.2011.09.0078)
摘
要
我国是葡萄属植物的主要起源地之一，也是世界葡萄属植物种类最多，遗传资源最为丰富的国家之一。迄今为止，
已知起源于我国的葡萄属植物共有
38
个种
1
个亚种和
7
个变种。如此丰富多彩的葡萄属种质资源不仅对葡萄科学的发展有
重大意义，也是阐明世界葡萄起源、演化和生物多样性必不可少的重要证据。利用核糖体转录间隔区
(ITS)
和
3
个叶绿体
DNA
(cpDNA)
序列
(
atpF-atpH
，
trnH-psbA
和
trnL-trnF
)
对
16
个中国葡萄野生种、变种及其近缘植物的
22
份材料的亲缘关系和分
子系统进化进行研究。以圆叶葡萄为外类群，首次利用
ITS
和
cpDNA
序列构建了中国野生葡萄的分子系统发育树。叶绿体
DNA
序列构建的系统发育树显示，
22
份材料之间的亲缘关系与其在不同大陆间的地理分布一致；
ITS
构建的系统发育树显
示，
22
份材料的
30
个克隆可分成
2
个主要分枝，其中分枝
E
又可分为
4
个亚分枝，由于多重核糖体
DNA
序列等因素影响，
ITS
树存在塌陷，
ITS
系统进化树结果较为复杂，仅能部分揭示
22
份材料之间的亲缘关系；综合分析
cpDNA
序列和
ITS
序
列的聚类结果，初步推断
‘
燕山葡萄
0947’
为母本为中国野生葡萄的一个种，父本为河岸葡萄的种间杂种；初步推断桑叶葡萄
为一个独立的中国葡萄野生种。
关键词
中国野生葡萄
;
核糖体转录间隔区
;
叶绿体
DNA
序列
;
亲缘关系
A Molecular Phylogenetic Study of Chinese Wild Vitis (Vitaceae) Based on
Internal Transcribed Spacer and Chloroplast DNA Sequence
Zhang Yonghui , Fan Xiucai , Zhang Ying , Sun Haisheng , Jiang Jianfu , Liu Chonghuai
Zhengzhou Fruit Research Institute ,The Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, P.R. China
Corresponding author, liuchonghuai@caas.net.cn;
Authors
Abstract
As one of the major original centers of Vitis species, China has abundant genetic resources in the world. So far, 38
species, 1 subspecies and 7 varieties of Chinese wild grapes were originated in China.The
vitis
germplasm resources were not only
significant in the development of grape science, but also provided an indispensable evidence to clarify the origin, evolution and
biodiversity of the Vitis in the world.The genetic relationship of 22
vitis
materials of 16 Chinese wild grape species and their relatives
were determined based on sequence analysis of nuclear internal transcribed spacer(ITS) and 3 chloroplast DNA (cpDNA)sequences
(
atpF-atpH
,
trnH-psbA
and
trnL-trnF
). Molecular phylogenetic trees were constructed based on cpDNA and ITS sequences with the
Vitis
rotundifolia as the outgroup. Based on the combined analysis of 3 chloroplastid DNA sequences,
Vitis
was split into three clades
that mirror the continental distribution of these accessions. Phylogenetic tree which was constructed by ITS sequence showed that
two main clades and four subclades were generated from the 22
vitis
materials. Due to the influence of Multiple rDNA arrays and
other facts, the phylogenetic of the ITS was relatively complex ,and only demonstrated the relationships of 22
vitis
materials partially.
From the clustering results of cpDNA sequences and ITS sequences, we preliminarily determined ‘Yanshanputao 0947’ was a
interspecific hybrid, which female and male parent were one species of the Chinese wild grapes and
V.
riparia
Michx., respectively.
V. heyneana
Roem.& Schult subsp.
ficifolia
(Bge.) C.L.Li. could be a separate species from this result.
Keywords
Chinese wild grape; ITS; Chloroplast DNA sequences; Genetic relationship
研究背景
我国是世界葡萄属
(
Vitis
L.)
植物种质资源最丰
富的国家，原产的葡萄属植物有
38
个种、
1
亚种和
7
个变种，尚不确定或有争议的疑问种或变种有
14