Page 24 - 2011no51

杨宁等, 2011, 利用分子标记辅助选育白菜 Y02 甲型“两用系 ”, 分子植物育种 Vol.9 No.51 (doi: 10.5376/mpb.cn.2011.09.0051)
1387
回交过程中会出现基因型不同的群体，表现型为可
育，利用分子标记从群体中选择基因型为 MsfMs 的
单株。选取基因型为 MsfMs 单株自交，自交后代兄
妹交以获得甲型“两用系”。
样本容量按公式 n≥lg (0.01) / lg (1- p) 计算，p为目
标个体出现概率；适合性测验取公式 X2
C = [ | A - ra
| - ( r +1)/2 ] 2/r.n。
作者贡献
杨宁、李跃飞和刘志勇是本研究的实验设计和实验研
究的执行人；杨宁完成数据分析，论文初稿的写作；李跃飞
和刘志勇参与实验设计，试验结果分析；冯辉是项目的构思
者及负责人，指导实验设计，数据分析，论文写作与修改。
全体作者都阅读并同意最终的文本。
致谢
本研究由国家自然科学基金(31071792) 和高等学校博
士学科点专项科研基金(20092103110001)共同资助。作者感
谢辽宁省十字花科蔬菜遗传育种重点实验室全体人员在本
实验过程中给予的技术支持和有益建议。感谢两位匿名同行
评审专家的评审建议和修改建议。
参考文献
Feng H., Wei P., Li C.Y., Choi S.R., Lin Y.P., and Piao Z.Y.,
2009, Identification of SSR markers linked to Ms, a genic
multiple-Allele male sterile gene in Chinese cabbage,
Yuanyi Xuebao (Acta Horticulturae Sinica), 36(1):
103-108 (冯辉, 魏鹏 , 李承彧, 崔水莲, 林容杓 , 朴钟
云, 2009, 大白菜核基因雄性不育复等位基因 Ms 的
SSR 标记, 园艺学报, 36(1): 103-108)
Feng H., Wei Y.T., and Ji S.J., 1998, Multiple Allele model for
genic male sterility in Chinese cabbage, Acta Hortic, 467:
133-142
Feng H., Wei Y.T., and Xu M., 1995, Genetic models for genic
male sterile line of Chinese cabbage and its verification,
The proceeding of horticulture of the secondly youth
science annual meeting of China association for science
and technology, Horticulture Dissertation, Beijing: Beijing,
Agricultural University Press, pp.458-466
Feng H., Xu W., and Wang Y.G., 2007, Directive transfer of the
genetic male sterile line of Milk Chinese cabbage AI023,
Yuanyi Xuebao (Acta Horticulturae Sinica), 34(3):
659-664 (冯辉, 徐巍, 王玉刚, 2007, ‘奶白菜 AI023’品
系核基因雄性不育系的定向转育, 园艺学报 , 34(3):
659-664)
Gao S.B., Carlos M., Debra J., Skinner A.F., Krivanek J.H.,
Crouch, and Xu Y.B., 2008, Development of a seed
DNA-based genotyping system for marker-assisted
selection in maize, Mol. Breed., 22: 477-494
Liang F.S., Deng Q.Y., Wang Y.G., Xiong Y.D., Jin D.M., Li
J.M., and Wang B., 2004, Molecular marker-assisted
selection for yield-enhancing genes in the progeny of
9311×O. rufipogon using SSR, Euphytica, 139: 159-165
Liu Z.Y., Feng H., Li C.Y., Wei P., and Wang L.L., 2010, SCAR
markers linked to Ms, a genetic male sterile gene in
Chinese cabbage, Yuanyi Xuebao (Acta Horticulturae
Sinica), 37(5): 757-762 (刘志勇 , 冯辉, 李承彧 , 魏鹏,
王丽丽, 2010, 与大白菜雄性不育基因 Ms 连锁的 SCAR
标记, 园艺学报, 37(5): 757-762)
Sang X.C., Yang Z.L., Zhong B.Q., Li Y.F., Hou L., Pei Y., Li
G.R., and He G.H., 2006, Assessment of purity of rice
CMS lines using cpDNA marker, Euphytica, 152: 177-183
Shen X.Q., and Yang W.J., 2004, RAPD marker linked to the
fertile restoring gene for the dominant genic male sterile
lines in Chinese cabbage, Yuanyi Xuebao (Acta
Horticulturae Sinica), 31(6): 731 (沈向群, 杨文俊, 2004,
大白菜核基因显性雄性不育性育性恢复基因的 RAPD
标记, 园艺学报, 31(6): 731)
Wang L.L., Wei P., Liu Z.Y., Li C.Y., Wang Y.G., Ji R.Q., and
Feng H., 2010, Genetic male sterile line of Chinese
cabbage developed by using molecular marker assisted
selection, Fenzi Zhiwu Yuzhong (Molecular Plant
Breeding), 8(2): 322-328 (王丽丽, 魏鹏, 刘志勇, 李承
彧, 王玉刚, 冀瑞琴, 冯辉, 2010, 利用分子标记辅助选
育大白菜核基因雄性不育系 , 分子植物育种, 8(2):
322-328)
Williamson V.M., Ho J.Y., Wu F.F., Miller N., and Kaloshian I.,
1994, A PCR-based marker tightly linked to the nematode
resistance gene, Mi, in tomato, Theor. Appli. Genet., 87:
757-63
Xin B., Feng H., Yang X.F., and Wang Y.G., 2009, Studies on
breeding for genetic male sterile line in Brassica rapa L.
ssp. Chinensis L.. Zhongguo Shucai (China Vegetables),
14: 38-42 (辛彬, 冯辉, 杨晓飞, 王玉刚, 2009, 青梗菜
核基因雄性不育系的转育, 中国蔬菜, 14: 38-2)
Yuan H., Zhang C.H., Liu H.H., Xuan S.X., Li X.F., and Shen
S.X., 2009, Chromosomal location and AFLP marker
screening of genic male sterile gene in Chinese cabbage,
Zhongguo Nongye Kexue (Scientia Agricultura Sinica),
45(6): 2061-2067 (袁鹤 , 张成合, 刘海河 , 轩淑欣 , 李
晓峰 , 申书兴 , 2009, 大白菜雄性核不育基因的染色体
定位及 AFLP 分子标记筛选, 中国农业科学, 42(6):