Basic HTML Version
栗婷等
, 2011,
落叶松体细胞胚胎全长
cDNA
文库构建及初步分析
,
分子植物育种
Vol.9 No.26 (doi: 10.5376/mpb.cn.2011.09.0026)
1195
400 μL LB
液体培养基，然后置于摇床上于
31
℃，
225 rpm
下培养
1 h
，之后取
5 μL
感染的细菌培养
物涂布到
LB/Carb
平板上，
37
℃下培养待长出单克
隆，取细菌单克隆于
LB
液体培养基中培养，送
invitrogen
公司测序。
测序获得的序列剔除载体序列、引物序列和
PolyA
后转换成
FASTA
格式文件输出，利用
contigexpress
软件进行序列的拼接，参数为程序默
认值，拼接后得到一致序列，包括由多条
ESTs
拼
接成的跨叠群
(contigs)
及单一序列
(singleton)
。用
Blastn
和
Blastx
对所得序列进行比对、查询和注释。
作者贡献
栗婷和朱彩虹是本研究的实验设计和实验研究的执行
人；栗婷完成数据分析，论文初稿的写作；齐力旺参与实验
设计，试验结果分析；韩素英是项目的构思者及负责人，指
导实验设计，数据分析，论文写作与修改。全体作者都阅读
并同意最终的文本。
致谢
本研究由国家
“973”
项目
(2009CB119100)
，国家自然科
学基金重点项目
(30830086)
，国家
“863”
项目
(SQ2010AA
1000687004, 2006AA100109, 2008AA10Z126)
和国家林业局
948
项目
(2007
-
4
-
03)
资助。
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