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曾兴莹等
, 2011,
花椰菜与黑芥体细胞杂种不同世代甲基化变异
MSAP
分析
,
分子植物育种
Vol.9 No.20 (doi: 10.5376/mpb.cn.2011.09.0020)
157
表
3 MSAP
分析的接头和引物序列
Table 3 MSAP adapters and primers used in this study
接头
Adapters
Eco
R
Ⅰ
-
adaptors
Ⅰ
5'
-
CTCGTAGACTGCGTACC
-
3'
Eco
R
Ⅰ
-
adaptors
Ⅱ
5'
-
AATTGGTACGCAGTC
-
3'
Hpa
Ⅱ
//
Msp
Ⅰ
-
adaptors
Ⅰ
5'
-
GATCATGAGTCCTGCT
-
3'
Hpa
Ⅱ
//
Msp
Ⅰ
-
adaptors
Ⅱ
5'
-
CGAGCAGGACTCATGA
-
3'
预扩引物
(5'→3')
Preselective primers (5'→3')
Eco
R
Ⅰ
+A
5'
-
GACTGCGTACCAATTCA
-
3'
Hpa
Ⅱ
//
Msp
Ⅰ
+0
5'
-
ATCATGAGTCCTGCTCGGT
-
3'
MSAP
选扩引物组合
(5'→3')
Selective primer combinations used in MSAP (5'→3')
Eco
R
Ⅰ
-AAC+
Hpa
Ⅱ
/
Msp
Ⅰ
-TCA
Eco
R
Ⅰ
-ACA+
Hpa
Ⅱ
/
Msp
Ⅰ
-TCA
Eco
R
Ⅰ
-AAC+
Hpa
Ⅱ
/
Msp
Ⅰ
-ATA
Eco
R
Ⅰ
-ACA+
Hpa
Ⅱ
/
Msp
Ⅰ
-ATA
Eco
R
Ⅰ
-AAC+
Hpa
Ⅱ
/
Msp
Ⅰ
-ACT
Eco
R
Ⅰ
-ACA+
Hpa
Ⅱ
/
Msp
Ⅰ
-ACT
Eco
R
Ⅰ
-AAC+
Hpa
Ⅱ
/
Msp
Ⅰ
-CAT
Eco
R
Ⅰ
-ACA+
Hpa
Ⅱ
/
Msp
Ⅰ
-CAT
Eco
R
Ⅰ
-AAG+
Hpa
Ⅱ
/
Msp
Ⅰ
-TCA
Eco
R
Ⅰ
-AAC+
Hpa
Ⅱ
/
Msp
Ⅰ
-TCA
Eco
R
Ⅰ
-AAG+
Hpa
Ⅱ
/
Msp
Ⅰ
-ATA
Eco
R
Ⅰ
-ACT+
Hpa
Ⅱ
/
Msp
Ⅰ
-ATA
Eco
R
Ⅰ
-AAG+
Hpa
Ⅱ
/
Msp
Ⅰ
-ACT
Eco
R
Ⅰ
-ACT+
Hpa
Ⅱ
/
Msp
Ⅰ
-ACT
Eco
R
Ⅰ
-AAG+
Hpa
Ⅱ
/
Msp
Ⅰ
-CAT
Eco
R
Ⅰ
-ACT+
Hpa
Ⅱ
/
Msp
Ⅰ
-CAT
作者贡献
刘凡老师、王桂香老师是本研究的实验设计和实验研
究的执行人；在此研究中曾兴莹完成了论文初稿的写作、数
据的分析以及结果分析。而且王桂香老师对此论文进行了修
改。全体作者都阅读并同意最终的文本。
致谢
本研究由北京市科技计划项目
(D0807050069
-
0803)
和
北京市科技计划项目
(Z09090501040902)
共同资助。
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