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曾兴莹等
, 2011,
花椰菜与黑芥体细胞杂种不同世代甲基化变异
MSAP
分析
,
分子植物育种
Vol.9 No.20 (doi: 10.5376/mpb.cn.2011.09.0020)
1154
表
2
甲基化模式在亲本和子代间的遗传与变异
Table 2 Methylation patterns in parents and offspring heredity and variation
位点类型
type
of
loci
黑芥
Brassica
nigra
花椰菜
Brassica ol-
eracea
L.
var
.
botrytis
L.
子代
offspring
单态性位点
Monomorp-hic
sites
Sitetape H M H M
H M
BC1
BC2
BC3
S2
S3
S4
非甲基化
Non-methylated
sites
X
1 1
1 1
1 1
117
(29.2%)
117
(28.2%)
117
(29.2%)
109
(27.2%)
114
(28.4%)
115
(28.7%)
A1
0 1
0 1
0 1
9(2.2%) 11(2.7%) 12(3.0%) 12(3.0%) 15(3.7%) 15(3.7%)
甲基化
Methylation sites A2
1 0
1 0
1 0
3(0.7%) 4(1.0%) 5(1.2%) 4(1.0%) 5(1.2%) 5(1.2%)
多态性位点
Polymorphic sites
B1
0 1
0 0
0 1
25
28
26
28
27
25
B2
0 0
1 1
0 0
43
44
45
42
42
41
B3
0 0
0 1
0 0
34
36
34
33
34
35
B4
1 1
0 1
1 1
25
23
25
26
22
23
B5
1 1
1 0
1 1
16
13
12
12
12
14
B6
1 0
1 1
1 0
11
12
14
12
14
13
B7
0 1
1 1
0 1
13
12
11
11
14
13
B8
0 0
1 0
0 0
13
14
15
14
15
15
B9
1 0
0 0
1 0
1
1
1
2
1
1
B10
1 1
0 0
1 1
1
1
1
1
1
1
遗传黑芥
Parental genetic
nigra
B11
1 0
0 1
1 0
2
6
2
4
2
1
Total
194
(48.4%)
190
(47.4%)
186
(46.4%)
185
(46.1%)
184
(46.0%)
182
(45.4%)
B12
0 1
1 1
1 1
4
1
1
3
5
3
B13
1 0
0 0
0 0
3
9
7
3
4
2
B14
0 0
0 1
0 1
0
7
10
7
9
8
B15
1 1
0 1
0 1
2
6
5
1
6
7
B16
0 1
0 0
0 0
11
9
8
9
6
7
B17
1 1
0 0
0 0
5
3
6
3
2
3
遗传花椰菜
Parental genetic
oleracea
B18
0 0
1 1
1 1
5
3
6
3
2
3
Total
29(7.2%) 36(9.0%) 38(9.5%) 30(7.5%) 37(9.2%) 35(8.7%)
C1
0 1
0 1
0 0
22
10
11
15
6
11
C2
1 1
1 1
0 1
7
10
9
6
4
3
C3
1 0
1 1
0 1
0
1
1
0
1
1
C4
1 1
1 0
0 1
0
3
1
1
2
1
C5
1 1
0 0
0 1
1
2
2
6
3
1
过甲基化位点
C1 hypermethyla-
tion
C6
1 1
1 1
0 0
12
10
11
11
12
15
Total
42(10.5%) 36(9.0%) 35(8.7%) 39(9.7%) 28(7.0%) 32(8.0%)
D1
0 0
0 0
1 0
2
3
3
5
4
5
D2
0 1
0 1
1 1
0
1
1
5
4
5
D3
0 1
0 0
1 1
2
0
0
3
3
2
D4
0 0
0 0
1 1
2
1
1
4
3
2
D5
0 0
0 1
1 1
1
2
2
2
2
2
去甲基化位点
D1 demethylation
D6
1 0
0 1
1 1
0
2
1
3
2
1
Total
7(1.7%) 9(2.2%) 8(2.0%) 22(5.5%) 18(4.5%) 17(4.2%)
Total
401
(100%)
401
(100%)
401
(100%)
401
(100%)
401
(100%)
401
(100%)