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陈深广等
, 2011,
利用分子标记鉴定超级稻恢复系
R9308
的白叶枯病抗性基因
,
分子植物育种
Vol.9 No.15 (doi: 10.5376/mpb.cn.2011.09.0015)
1110
2
用于抗性基因检测的分子标记
Table 2 Molecular markers used for assay of bacterial blight resistance genes
标记
Marker
前引物
(5'
-
3')
Forward primer (5'
-
3')
后引物
(5'
-
3')
Reverse primer (5'
-
3')
参考文献
Reference
Xa4
ATCGATCGATCTTCACGAGG
TGCTATAAAAGGCATTCGGG
Huang et al., 1997
xa5
TAGCTGCTGCCGTGCTGTGC
AATATTTCAGTGTGCATCTC
Iyer et al., 2004
Xa7
CGATCTTACTGGCTCTGCAACTCTGT GCATGTCTGTGTCGATTCGTCCGTACGA Porter et al., 2003
xa13
AGCTCCAGCTCTCCAAATG
GGCCATGGCTCAGTGTTTAT
Chu et al., 2003
Xa21
ATAGCAACTGATTGCTTGG
CGATCGGTATAACAGCAAAAC
Yoshimura et al., 1998
作者贡献
陈深广和杨杨是本研究的实验设计和实验研究的执行
人,也是数据分析,论文初稿的完成人;吴建利和程式华是
项目的构思者及负责人,指导实验设计,数据分析,论文写
作与修改。全体作者都阅读并同意最终的文本。
致谢
本研究由国家
863
计划专项
(2009AA101101)
和农业部
超级稻专项
(2006
-
9)
资助。感谢国际水稻研究所
VeraCruz
士提供白叶枯病菌小种。
参考文献
Cao L.Y., Zhuang J.Y., Yuan S.J., Zhan X.D., Zheng K.L., and
Cheng S.H., 2003, Hybrid rice resistant to bacterial leaf
blight developed by marker assisted selection, Rice Sci.,
11: 68-70
Chu Z.H., Yuan M., Yao J.L., Ge X.J., Yuan B., Xu C.G., Li
X.H., Fu B.Y., Li Z.K., Bennetzen J.L., Zhang Q.F., and
Wang S.P., 2006, Promoter mutations of an essential gene
for pollen development result in disease resistance in rice,
Genes Dev., 20: 1-5
Gu K.Y., Yang B., Tian D., Wu L., Wang D., Sreekala C., Yang
F., Chu Z., Wang G.L., White F.F., and Yin Z., 2005, R
gene expression induced by a type-III effector triggers
disease resistance in rice, Nature, 435: 1122-1125
Huang N., Angels E.R., Domingo J., Mangpantay G., Singh S.,
Zhang G., Kumar N., Vadivel B.J., and Khush G.S., 1997,
Pyramiding of bacterial blight resistance genes in rice:
marker-assisted selection using RFLP and PCR, Theor
Appl Genet, 95: 313-320
Iyer A.S., and McCouch S.R., 2004, The rice bacterial blight
resistance gene
xa5
encodes a novel form of disease
resistance, Mol. Plant Microbe Int., 17: 1348-1354
Ji G.H., Zhang S.G., and Xu Z.G., 2000, Evaluation on
resistance of rice cultivars to bacterial blight, Yunnan
Nongye Daxue Xuebao (Journal of Yunnan Agricultural
University), 15(4): 321-323 (
姬广海
,
张世光
,
许志刚
,
2000,
水稻品种对白叶枯病抗性鉴定的研究
,
云南农业
大学学报
, 15(4): 321-323)
Jin X.W., Wang C.L., Yang Q., Jiang Q.X., Fan Y.L., Liu G.C.,
and Zhao K.J., 2007, Breeding of near-isogenic line CBB30
and molecular mapping of
Xa30
(
t
), a new resistance gene
to bacterial blight in rice, Zhongguo Nongye Kexue (Sci.
Agric. Sin.), 40(6): 1094-1100 (
金旭炜
,
王春莲
,
杨清
,
江祺祥
,
樊颖伦
,
刘古春
,
赵开军
, 2007,
水稻抗白叶枯
病近等基因系
CBB30
的培育及
Xa30
(
t
)
的初步定位
,
国农业科学
, 40(6): 1094-1100)
Lu Y.J., and Zheng K.L., 1992, A simple method to extract rice
DNA, Zhongguo Shuidao Kexue (Chin. J. Rice Sci.), 6(1):
47-48 (
卢扬江
,
郑康乐
, 1992,
提取水稻
DNA
的一种简
易方法
,
中国水稻科学
, 6(1): 47-48)
Porter B.W., Chittoor J.M., Yano M., Sasaki T., and White F.F.,
2003, Development and mapping linked to the rice bacterial
blight resistance gene
Xa7
, Crop Sci., 43: 1484-1492
Shen W.W., Song C.L., Chen J., Fu Y.P., Wu J.L., and Jiang S.M.,
2010, Transgenic rice plants harboring genomic DNA from
Zizania latifolia
(Griseb.) confer bacterial blight resistance,
Zhongguo Shuidao Kexue (Chin. J. Rice Sci.), 24(5): 447-452
(
沈玮玮
,
宋成丽
,
陈洁
,
付亚萍
,
吴建利
,
江绍玫
, 2010,
转菰候选基因克隆获得抗白叶枯病水稻植株
,
中国水
稻科学
, 24(5): 447-452)
Song W.Y., Wang G.L., Chen L.L., Kim H.S., Pi L.Y., Holsten
T., Gardner J., Wang B., Zhai W.X., Zhu L.H., Fauquet C.,
and Ronald P., 1995, A receptor kinase-like protein encoded
by the rice disease resistance gene,
Xa21
, Science, 270:
1804-1806
Sun X.L., Cao Y.L., Yang Z.F., Xu C.G., Li X.H., Wang X.P.,
and Zhang Q.F., 2004,
Xa26
, a gene conferring resistance
to
Xanthomonas oryzae
pv.
oryzae
in rice, encoding an
LRR receptor kinase-like protein, Plant J., 37: 517-527
Wang C.L., Zhao B.Y., Zhang Q., Zhao K.J., and Xing Q.D., 2004,
Identification of a new rice germplasm with resistance to
bacterial blight and the breeding of a near-isogenic line,
Zhiwu Yichuan Ziyuan Xuebao (Journal of Plant Genetic