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Legume Genomics and Genetics 2012, Vol.3, No.3, 14
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17
Figure 3 Location of the QTLs for the trait of the days of full
maturity on chromosome 6 and chromosome 18 in the JINF
RIL population
ZDD2315)בJidou 23’ (
Glycine max
Merr., CV. Jinbean
23) and a pod shattering susceptible cultivar ‘Huibuzi’.
In present mapping study, we found more than one
QTLs located on soybean molecular linkage group C2
(chromosome 6), and a major QTL for PD (
qPDH6
-
1
)
located on Chr. 6 with the genetic distances ranging
from 0.4 cM to 3.6 cM, a major QTL for DEM
(
qTFM6
-
1
) located from 0.1 cM to 7.3 cM on Chr. 6.
Both QTLs were located in the region between
Satt
_
062
and
Satt520
. In addition, a major QTL for
DFM (
qDFM6
-
1
) was located on Chr. 6 with the
genetic distance from 16.4 cM to 28.7 cM. The above
3 QTLs showed overlapping or adjacent position
indicating that there might be many candidate genes
controlling PD located on the Chr.6 in soybean.
Tsuchiya (1987) reported that the ratio of width to
thickness of the resistant varieties was higher than
those of the susceptible varieties, and the size and
shape of pods may not directly related to PD.
Caviness’s (1969) study showed that the value of
width/thickness and PD were related to seed size.
In this study, there was a significantly negative
correlation among PD, the value of width/thickness
and date of maturity which confirmed the results from
previous researches.
Arnaud and his colleague’s study showed that
synthesis of the phytohormone gibberellin has a key
role in patterning the Arabidopsis fruit and it is
required for fruit opening (Arnaud et al., 2010). Based
on this result and analysis of bioinformatics of
mapped QTLs, we found a gene
Glyma06g00860.1
with protein activity in gibberellin response located on
Chr. 6 near the
qPDH6
-
1
, and a gibberellin transfer
protein gene
Glyma04g43150.1
located on Chr. 4 near
the
qRTW4
-
1
. Although it was unclear what the
functional annotations of these two predicted genes
were and what their protein products were, we
suggested that gibberellins and its relative synthesis
genes might play a key role in PD process of soybean.
Christiansen et al (2002) found that the genes
encoding for the polygalacturonase (PG) were different
between soybean and rapeseed, but Petersen et al.,
(1996) demonstrated that the polygalacturonase was
related to PD in rapeseed. Coincidentally, we found
two polygalacturonase genes encoding for activity
protein, which were
Glyma04g43340.1
l near the
qRTW4
-
1
on chromosome 4 and
Glyma18g16870.1
near the
qDFM18
-
1
on chromosome 18, respectively.
This result implied that PG might play a role in the PD
process of soybean, and it should be a new direction
for further research.
Scientists thought that hydrolase plays a key role in
the process of PD (Fry et al., 1992; Agrawal et al.,
2002), and we found a gene
Glyma06g01500.1
encoding for the hydrolytic enzymes located on Chr. 6
near to the location of
qPDH16
-
1
.
Although the functional annotation of above predicted
genes, and whether their protein products related to
PD remained unclear, we predicted that gibberellins,
relevant PG and hydrolytic enzymes might play a
different role in PD regulatory network in plant. In
addition, Arnaud et al (2011) showed that it would be
helpful to regulate fruit opening and seed dispersal by
changing the gene sequence of promoters, which