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Legume Genomics and Genetics 2012, Vol.3, No.3, 14
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16
Figure 1 Location of the QTL for PD on chromosome 6 in the
JINF RIL population
the LG A2 (chromosome 8) with LOD value 2.14 and
genetic distance of 3.7 cM, and it accounted for 7.02%
of the total variance.
1.4 QTL mapping of DFM
We also identified the QTL location for DFM in 112
soybean strains of JINF population by the composite
interval mapping (CIM) method in WinQTLCart 2.5.
Setting The LOD value>2.0, we acquired three QTLs
for DFM, which were designated as
qDFM6
-
1
,
qDFM6
-
2
and
qDFM18
-
1
(Figure 3; Table 1).
Both
qDFM6
-
1
and
qDFM6
-
2
were located on the
linkage group C2 (chromosomes 6). They were
located in the region between Sat_062 and Satt520,
and between Satt291 and Satt305, with the LOD
values 6.59 and 2.85, the genetic distances of 7.2 cM
and 12.3 cM, and accounted for 21.77% and 9.74% of
the variance, respectively.
qDFM18
-
1
was located in
the region between markers Satt217 and Satt130 on
the linkage group G (chromosome 18) with the LOD
value 2.81, the genetic distance of 0.6 cM, and
accounted for 7.74% of the variance.
1.5 Analysis of genomic information of related
QTLs
Based on the Genome ANNOTATION INFORMATION
of soybean on http://www.phytozome.net/cgi-bin/
gbrowse/soybean/, we found a gene that have the
auxin response protein activity,
Glyma06g00860.1
,
and a gene encoding hydrolytic enzyme protein,
Glyma06g01500.1
, near the location of
qPDH6
-
1
. We
also found a gene encoding auxin transfer protein,
Glyma04g43150.1
, and another gene encoding
endo-polygalacturonic acid enzyme,
Glyma04g43340.1
,
near the location of
qRTW4
-
1
. There was also an
endo-polygalacturonic acid enzyme gene,
Glyma18g-
16870.1
, near the location of
qDFM18
-
1
.
2 Discussion
Most previous studies suggested that the major QTL
(
qPDH1
) for PD in soybean was mapped on soybean
molecular linkage group J (chromosome 16) (Funatsuki
et al., 2006; 2008; Liu et al., 2007; Kang et al., 2009;
Suzuki et al., 2010). Kang et al (2009) reported that
different breeding strategies will be required for PD
depending on different genetic backgrounds (Kang et
al., 2009). Our present research result showed that
there was discrepancy from previous studies in QTL
mapping, and the major QTL (
qPDH6
-
1
) was mapped
on chromosome 6. This is probably due to the testing
materials in the study, RIL population derived from
across between ‘Huibuzi’ (
Glycine max
Merr., CV.
Figure 2 Location of three QTLs for the ratio of thickness to width on chromosome 11, 4 and 8 in the JINF RIL population