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42
Albania, Bulgaria, the former Yugoslavia, Ukraine,
Georgia, Tacikistan, Iran, and Pakistan was reported
(Whitehouse, 2004; Chinikar et al., 2004; Papa et al.,
2004).
The enzyme-linked immunosorbent assay (ELISA)
was a highly sensitive and specific tool for CCHF
diagnosis (Garcia et al., 2006).
CCHV is a zoonotic disease that affect people who
come into contact with livestock and tick and
in
endemic areas, animals infection appear to be one of
the best indicators of risk to human infection (Kuljic-
Kapulica, 2004 ).
4 Conclusion
We conclude from this report that rapid diagnosis of
CCHF is important to prevent the spread of CCHF
virus among the health people.
5 Materials and Methods
Peripheral blood sample was collected at the onset of
the disease using Vacutainer tubes. Blood sample were
centrifuged at 1 500 rpm for 30 min, serum were
separated, transferred into 2 mL Eppendorf micro-
centrifuge tubes and stored at –20
℃
until used for the
ELISA.
Detection of CCHFV antigen in serum samples was
performed with the use of a commercially kit
according to the manufacturer’s recommendations.
Standardized optical density (OD) values were
calculated as follows: standardized OD = (raw OD of
sample
－
raw OD of negative control) / (raw OD of
positive control
－
raw OD of negative control).
Values < 0.22 were considered negative, values > 0.35
positive (+) and values > 0.35 strong positive (++).
Samples with OD values from 0.22 to 0.40 were
retested with another serum.
Acknowledgements
I would like to thank the University of Istanbul for their
supporting.
References
Bosan A.H., Kakar F., Dil A.S., Asghar H., and Zaidi S., 2000,
Crimean-Congo hemorrhagic fever (CCHF) in Pakistan,
Dis Surveil, 2: 4-5
Burt F.J., Swanepoel R., Shieh W.J., Smith J.F., Leman P.A.,
Greer P.W., Coffield L.M., Rollin P.E., Ksiazek T.G.,
Peters C.J., and Zaki S.R., 1997, Immunohistochemical
and in situ
localization of Crimean-Congo hemorrhagic
fever (CCHF) virus in human tissues and implications for
CCHF pathogenesis, Arch. Pathol. Lab. Med., 121: 839-
846 PMid:9278612
Carhan A.Y., Ozkaya E., and Ertek M., 2008, A preliminary
report on Crimean-Congo haemorrhagic fever in Turkey,
March - June, Euro. Surveil., 13: 18953 PMid:18761892
Chinikar S., Persson S.M., Johanson M., Bladh L., Goya M.,
Housmand B., Mirazimi A., Plyusnin A., Lundkvist A.,
and Nilsson M., 2004, Genetic analysis of Crimean-Congo
Hemorrhagic Fever Virus in Iran, J. Med. Virol., 73: 404-
411 http://dx.doi.org/10.1002/jmv.20106 PMid:15170636
Flick R., and Whitehouse C.A., 2005, Crimean-Congo
hemorrhagic fever virus, Curr. Mol. Med., 5: 753-760
http://dx.doi.org/10.2174/156652405774962335 PMid:16375710
Garcia S., Chinikar S., Coudrier D., Billecocq A., Hooshmand
B., Crance J.M., Grain D., and Bouloy M., 2006,
Evaluation of Crimean-Congo Hemorrhagic Fever virus
recombinant antigen expressed by Semliki Forest suicide
virus for IgM and IgG antibody detection in human and
animal sera collected in Iran, J Clin Virol., 35(2):154-159
http://dx.doi.org/10.1016/j.jcv.2005.02.016 PMid:16087395
Kuljic-Kapulica N., 2004, Emerging diseases Crimean-Congo
Hemorrhagic Fever Virus (in Serbian), Med Pregl, 57 (9-
10):354-356 http://dx.doi.org/10.2298/MPNS0410453K
PMid:15675618
Papa A., Christova I., Papadimitriou E., and Antoniadis A.,
2004, Crimean-Congo hemorrhagic fever in Bulgaria,
Emerg. Infect. Dis., 8:1465-1467
http://dx.doi.org/10.3201/eid1008.040162
PMid:15496250 PMCid:3320408
Prajapati D.S., Patel K.R., Sen D.J., Patel S.J., and Grag C.S.,
2011, Crimean-Congo hemorrhagic fever From Tick-
Borne Viral Disease, Pharmacie globale, IJCP, 3(02):1-6
Swanepoel R., Shepherd A.J., Leman P.A., Shepherd S.P.,
McGillivray G.M., Erasmus M.J., Searle L.A., and Gill
D.E., 1987, Epidemiologic and clinical features of
Crimean-Congo hemorrhagic fever in southern Africa, Am
J Trop Med Hyg., 36(1):120-132 PMid:3101525
Whitehouse C.A., 2004, Crimean-Congo hemorrhagic fever,
Antiviral Res., 64:145-160
http://dx.doi.org/10.1016/j.antiviral.2004.08.001 PMid:15550268
Wilson M.L., and Digoutte M., 1990, Ecology and
epidemiology of Criman-Congo haemorrhagic fever virus
transmission in the Republic of Senegal, Annual Report,
Institute Pasteur, Dakar, Senegal