Basic HTML Version
林茂等
, 2011,
甘蓝型油菜隐性核不育系及其
DH
系的纯度的
SSR
检测
,
分子植物育种
Vol.9 No.6 (doi: 10.5376/mpb.cn.2011.09.0006)
1047
之间的遗传距离小于姊妹交后代之后的遗传距离。
2
讨论
细胞质雄性不育
(CMS)
和细胞核雄性不育
(GMS)
是甘蓝型油菜杂种优势利用的两条主要途径。
在细胞质雄性不育中，波里马细胞质雄性不育
(pol
CMS))
是目前国内外最有实用价值的细胞质雄性不
表
1 SSR
分析所用引物序列及扩增结果
Table 1 The primer alignment and amplified results of SSR analysis
编号
Number
名称
Name
正向引物
Forward primer
反向引物
reverse primer
多态性带
polymorphism
325
CB10022
5'
-
AACAACCAAACATAGTCCC
-
3'
5'
-
GTTGACTTTGACCTTGACTT
-
3'
2
390
CB10364
5'
-
GAGACGATGCAAAGATCG
-
3'
5'
-
TGCAGACACATTCGAACA
-
3'
2
391
CB10369
5'
-
CATTCACAGGACCAGAGC
-
3'
5'
-
CAAAGCCAAGACAACCAT
-
3'
1
599
sS2331B
5’
-
TGTCCTGTTTTCTGTGCTGG
-
3'
5'
-
GCCAACGCTAGTTTTGCTTC
-
3'
3
621
sN11516
5’-GCGATCTCCTCAGGCATAGT
-
3'
5'
-
CCACGCAAGCTGAAACATAA
-
3'
2
624
sNRD03
5’
-
GAAGATTCGAGCTCTTTCGG
-
3'
5'
-
CGTTTCAGAATCATATTGTATTTTGCT
-
3'
2
625
sORF73
5'
-
CGTGGGCCAAGCTTAGATTA
-
3'
5'
-
CGTTCAAGAAGACACAGATCAAA
-
3' 2
626
sN12353
5'
-
CCGGCTTGGTTCGATACTTA
-
3'
5'
-
TTGCGAATCTTTAAGGGACG
-
3'
2
627
sR9222
5'
-
CACCGAACAAAACTGAGGGT
-
3' 5'
-
CGTTTCACTGCGTTCTACCA
-
3'
2
639
sR7223
5'
-
AGGACCCGACTTTCCTTGTT
-
3'
5'
-
ACCAAACTCGGCGTACAAAT
-
3'
1
650
sR9447
5'
-
AAATTCGAAAATGCAAACGG
-
3' 5'
-
CCAATCTTGGAACAATAGAAGATG
-
3' 2
652
sR9251J
5'
-
TCTCCAGTACGGGGAAGAGA
-
3' 5'
-
CAAAGAAGCCATGTGTGGAA
-
3'
2
653
sR12777
5'
-
CAAGCAGTTTAAGGAACCGC
-
3' 5'
-
ATAATTGCATTTTGCTCCGC
-
3'
2
697
BRMS071
5'
-
CAAAGCGAGAAAGTGCAGTTGA
GAG
-
3'
5'
-
TCCACGAAACTACTGCAGATTGAAA
-
3'
2
788
cnu_ssr050
5'
-
AGCCCAAGCTCGTATTCCTT
-
3'
5'
-
AAAATCGGGACAACCACCTA
-
3'
0
792
cnu_ssr090
5'
-
GCAAAGATCGGCGAAGAAGA
-
3' 5'
-
TGCAGACACATTCGAACAAACA
-
3'
0
799
KBRH139B23 5'
-
ATCTCATGGTTGGTTCACCG
-
3'
5'
-
ATTTCCAAAACACACACGCA
-
3'
1
810
EJU4
5'
-
CACCTTATCATCTCTCTATCCC
-
3' 5'
-
CCTCTGTTTCTCTCCTTGTG
-
3'
1
811
EJU5
5'
-
GGCACGTACATGGAGGATTC
-
3'
5'
-
TGTTGGTCGAGCTGTTTCAG
-
3'
1
821
ENA19
5'
-
AAGTTACCAAGGAGAGGACAG
-
3' 5'
-
AAAGGGACGCTACAAGTCA
-
3'
1
图
2
引物
821
在
DH
植株和姊妹交后代的指纹图谱
注
:
泳道
1,3,5,…45,47
是胚状体苗
; 2,4,6,…46,48
是姊妹交后代
Figure 2 Amplified profile by SSR primer (821) between the DH plants and first-cousin cross offspring
Note: The offspring increased in odd path of electrophoresis from DH plants and in even path of electrophoresis from first-cousin
cross offspring