Basic HTML Version
谢树章等
, 2011,
内含子调控转基因植物外源基因的表达研究
,
分子植物育种
Vol.9 No.12 (doi: 10.5376/mpb.cn.2011.09.0012)
1088
续表
1
Continuing table 1
Rice a-tubulin first
introns
GUS
水稻
Rice
增强基因的表达量
Elisa Fiume (2004)
内含子
Intron
目的基因
Aim gene
转化受体
Transformation
receptor
结果情况
Result
研究者
Investigator
The first introns
from both the
TRP1 and UBQ10
GUS
拟南芥
Arabidopsis
thaliana
内含子提高转录和促进翻译是两种不同的
机制
Introns increase transcription and promote
translation by two distinct mechanisms
Alan B. Rose (2004)
The first intron of
rice EPSP
GUS
烟草
Tobacco
增强基因的表达
Xu Junwang (xu et al.,
2003)
The sense
orientation of the
PClSV leader
sequence
GUS, CAT
烟草
Tobacco
提高了基因的表达量
evel
Bhattacharyya Somnath
(2003)
Ubi.U4 leader
intron
GUS
烟草
Tobacco
提高基因的表达量
,
而且能够调控基因的
组织特异性表达
Enhanced gene
nd
expression position
Plesse B. (2001)
Introns of
replacement
histone H3 genes
GUS
拟南芥
Arabidopsis
thaliana
增强基因表达量
2
-
70
倍
anging from
2
-
fold to 70
-
fold
Chaubet-Gigot N.
(2001)
Maize ubi1,
maize adh1,
rice act1 and
sugarcane rbcS
genes
BT6.1 promoter
香蕉
Banana
Rice act1
内含子提高
BT6.1
启动子活性
300
倍
, maize ubi1
提高
100
倍
, sugarcane
rbcS intron
提高
10
倍
, maize adh1
没有明
显的影响
The rice act1 and maize ubi1 introns
increasing native BT6.1 promoter activity by
about 300
-
fold and 100
-
fold, respectively.
The sugarcane rbcS intron increased
expression about tenfold, whereas the adh1
intron had no significant effect
Dugdale B. (2001)
Potato ST-LS1,
pea legumin
introns
GUS
烟草
Tobacco
内含子及其插入位点对基因的表达很关键
Introns and their sites of insertion into gene
constructs were important to gene
expression
IbrahimA.F.M. (2001)
PIV2
GFP
烟草、玉米
Tobacco, maize
能有效调控单子叶和双子叶植物的表达
Indicating that the PIV2 intron is spliced
effectively in both monocotyledonous and
dicotyledonous plant species
Mankin S. L. (2001)
TPI
GUS
烟草
Tobacco
提高报告基因
gusA
的表达水平，呈现出
明显的增强子特性
evel,
Showed a significant characteristic enhancer
谢先芝等
(2001)
Maize ubi1
promoter, exon
and intron
GUS
麦类作物幼穗
Tritordeum and
wheat
表达量是对照的
50
倍
y 50
-
fold
Sancha Salgueiro
(2000)