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谢树章等
, 2011,
内含子调控转基因植物外源基因的表达研究
,
分子植物育种
Vol.9 No.12 (doi: 10.5376/mpb.cn.2011.09.0012)
1087
续表
1
Continuing table 1
内含子
Intron
目的基因
Aim gene
转化受体
Transformation
receptor
结果情况
Result
研究者
Investigator
Ubi1 intron
Bt cry1Ah
玉米
Maize
增强基因表达
20%
左右
Showed that the expression of Cry1Ah
protein in the construct containing the ubi1
intron (pUUOAH) was 20% higher than that
of the intronless construct (pUOAH)
Wang YueBing (2008)
AGAMOUS
second intron
GUS
拟南芥
Arabidopsis
thaliana
植株不育
f transgenic plants
Liu Z.R.(2008)
LeSUT1 introns
GUS, Tomato
LeSUT1
烟草
Tobacco
内含子调控基因组织特异性表达。
LeSUT1
第二内含子促进基因在伴胞和保卫细胞中
表达
, LeSUT1
第三内含子促进基因在表
皮毛中表达
Showed three introns remarkable functions
for SUT
Intron 3
is responsible for expression in trichomes,
whereas intron 2 is necessary for expression
in companion cells and guard cells
Weise Andreas (2008)
5'UTR intron of
the rubi3 gene
GUS
水稻
Rice
提高基因表达量
evel
Lu Jianli (2008)
AGAMOUS
second intron
Barnase
烟草
Tobacco
表现出更高的不育性
Showed higher sterility
Wang HuiZhong (2008)
Ubil, actl, adhl
和
SBgLR2
GUS
玉米
Maize
ubil
增强基因表达活性的能力最强
,
可使
GUS
的表达活性提高
5
倍
, actl
可使
GUS
的表达活性提高
3
倍
, adhl
和
SBgLR2
没有
增强
GUS
基因的表达活性
Ubil enhanced the GUS gene activity by
5-fold, actl enhanced GUS gene activity by
3-fold. Adhl and SBgLR2 cann’t enhance
GUS gene activity
王悦冰等
(2008)
The leader intron
of the Arabidopsis
Cox5c gene
GUS, Hahb-4
拟南芥
Arabidopsis
thaliana
增强基因的表达和抗逆性
and stress
tolerance
Cabello J V (2007)
Intron2 and
intron9 of GBSSI
cDNA
GBSSI cDNA
inverted repeat
constructs
马铃薯
Nicotiana
tabacum
两个内含子都对基因沉默不产生影响
The two introns did not result in
enhancement of silencing in experimental
system
Heilersig H J B (2006)
SeFAD2 introns
GUS
拟南芥
Arabidopsis
thaliana
提高
GUS
基因表达量
100
倍，内含子具有
启动子活性
Enhanced the GUS gene activity by
100-fold,the intron hav
Kim Mi Jung (2006)
Introns of actin
(Act) genes
rhVEGF
小立碗藓
Physcomitrella
patens
增强基因表达最高达到
7
倍
evel by 7
-
fold
Weise Andreas (2006)
Castor bean
catalase intron
C1 gene
番茄
Tomato
构建反义表达载体，抑制基因的表达
An intron–hairpin genetic construction to
induce post-transcriptional gene silencing
against the early TYLCV replication
associated protein gene (C1)
Alejandro Fuentes
(2006)