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分子植物育种(网络版)
,
2011
,
9
,
1001
-
1009
Fenzi Zhiwu Yuzhong (Online), 2011, Vol.9, 1001
-
1009
http://mpb.
5th
.sophiapublisher.com
1001
研究论文
Research Article
斑茅初级核心种质秩数加权平均取样策略
毛钧
1
,
刘新龙
1
,
苏火生
1
,
应雄美
1
,
陆鑫
1
,
马丽
1
,
范源洪
1
,
蔡青
1,2
1.
云南省农业科学院甘蔗研究所
/
云南省甘蔗遗传改良重点实验室
,
开远
, 661600
2.
云南省农业科学院生物技术与种质资源研究所
,
昆明
, 650223
通讯作者
:
caiqingysri@163.com;
作者
分子植物育种
, 2011
,
9
,
1
doi: 10.5376/mpb.cn.2010.09.0001
收稿日期:
2010
10
17
接受日期:
2010
11
29
发表日期:
2011
01
06
这是一篇采用
Creative Commons Attribution License
进行授权的开放取阅论文。只要对本原作有恰当的引用
,
版权所有人允许并同意第三方无条
件的使用与传播。
引用格式:
毛钧等
, 2011,
斑茅初级核心种质秩数加权平均取样策略
,
分子植物育种
Vol.9 No.1 (doi: 10.5376/mpb.cn.2011.09.0001)
本研究以国家甘蔗种质资源圃收集保存的
147
份斑茅野生资源为原始材料,选取
26
个质量和数量性状,采用秩数
加权分析的方法,从取样方法、分组原则、组内取样比例
3
个层次探讨构建斑茅初级核心种质的最佳取样策略。取样方法采
用聚类取样
(C)
和随机取样
(R)
;分组原则包括采集地分组
(O)
、叶型分组
(L)
、海拔分组
(H)
、气候区域分组
(Z)
、总体聚类分组
(C)
及不分组随机
(N)
;组内取样比例包括简单比例
(P)
、对数比例
(L)
、平方根比例
(S)
和多样性比例
(G)
9
个总体取样梯度
10%
15%
20%
25%
30%
35%
40%
45%
50%
,每梯度设
3
次重复。通过比较香农多样性指数、变异系数、表
型方差、表型频率方差和表型保留比例
5
个参数的秩数加权平均值来检验各取样策略的优劣。结果表明,斑茅初级核心种质
最佳取样策略为叶型分组、对数比例、聚类取样,最佳总体取样量为
35%
。在此基础上,加入缺失表型材料、优良亲本和
抗性材料共计
58
份组成最终的斑茅初级核心种质库,占总资源数的
39.46%
关键词
斑茅
;
核心种质
;
秩数
;
加权平均值
;
取样策略
Sampling Strategy of Weighted Average Rank for Pre-Core Germplasm in
Erianthus arundianceus
Mao Jun
1
, Liu Xinlong
1
, Su Huosheng
1
, Ying Xiongmei
1
, Lu Xin
1
, Ma Li
1
, Fan Yuanhong
1
, Cai
Qing
1,2
1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences / Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, 661600,
P.R. China
2. Biology & Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650231, P.R. China
Corresponding author, caiqingysri@163.com;
Authors
Abstract
In this study, 147 clones of Erianthus arundianceus from the National Nursery of Sugarcane Germplasm Resources
(NNSGR) in Kaiyuan China were selected as original materials. Based on 26 quantitative and qualitative traits, the best sampling
strategy was discussed by weighted average rank analysis method from 3 levels as follows: 2 sampling methods (random sampling
and cluster sampling); 6 grouping principles (original grouping, leaves grouping, height grouping, zone grouping, cluster grouping
and not grouping); 4 sampling ratios within group (simple ratios, logarithmic ratios, square root ratios and genetic ratios). At the
same time, 9 grades for total sampling percentages (10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% and 50%) were analyzed and each
grades included 3 repeats. The sampling strategies and the pre-core collection were evaluated by weighted average rank of five
effective parameters (Shannon diversity index, coefficient of variation, variance of phenotypic value, variance of phenotypic
frequency and ratio of retaining phenotypic traits). The result indicated that the best sampling strategy for Erianthus arundianceus
was set up based on leaves grouping, logarithmic ratio, cluster sampling and 35% total sampling percentage. And some manual
selected materials were put in the pre-core collection, which including the rare phenotypic trait clones, the elite parents or resistibility
clones. Finally, the pre-core collection of Erianthus arundianceus included 58 clones which accounted for 39.46% of total resources.
Keywords
Erianthus arundianceus
; Core germplasm; Rank; Weighted average; Sampling strategy
研究背景
斑茅
(
Erianthus arundinaceus
)
是“甘蔗复合体
(
Saccharum
complex)
”中重要的野生资源,具有丛
生性好、萌芽力强、分蘖多、生长势强、宿根性好、
适应性广、抗逆性强、抗病虫性强等优异性状,成
为现代甘蔗遗传育种和种质创新中利用较多的种