Basic HTML Version
分子植物育种
(
网络版
), 2010
年
,
第
8
卷
,
第
5
篇
Fenzi Zhiwu Yuzhong (Online), 2010, Vol.8, No.5
http://mpb.
5th
.sophiapublisher.com
共
8
页，第
6
页
3.5 LTQ
结果分析
LTQ
原始文件经由
BioWorks 3.0
软件组自带的
Sequest Cluster
进行查库分析，数据库来自
swissprot
(http://www.uniprot.org/taxonomy, Taxon identifier:
48736 proteome set for Ralstonia 5/27/2009 released)
，
查库参数简要为母离子宽容度
3 Da
，碎片离子宽容
度为
0.8 Da,
固定修饰设置半胱氨酸
(+57.02 Da)
，动
态修饰设置甲硫氨酸氧化
(+57.02 Da)
，胰蛋白酶最
多允许两个内部酶切位点。利用
BioWorks
生成
Ralstonia
正反库，筛选查库结果假阳性率为
0.05%
，
并且含有两个以上唯一肽段的蛋白，去除冗余数据
后进行统计学分析。
作者贡献
宋浩、丁伟、沙伟、刘翀、周燕是本研究的实验设计
和实验研究的执行人；丁伟和宋浩及沙伟完成数据分析，论
文初稿的写作；宋浩和丁伟参与实验设计，试验结果分析；
陈薇是项目的构思者及负责人，指导实验设计，数据分析，
论文写作与修改。全体作者都阅读并同意最终的文本。
致谢
本研究受湖南中烟工业公司科技计划项目的资助。作
者感谢中国农科院为本实验过程中提供实验菌株。感谢两位
匿名的同行评审人的评审建议和修改建议。本文中提到了我
们实验中涉及的有关试剂供应商和测序服务商，这并非我们
为这些试剂供应商和测序服务商的产品和服务提供推荐或
背书
。
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