分子植物育种
(
网络版
), 2010
年
,
第
8
卷
,
第
2
篇
Fenzi Zhiwu Yuzhong (Online), 2010, Vol.8, No.2
http://mpb.
5th
.sophia
p
ublisher.com
共
5
页,第
5
页
作者贡献
刘鑫是本研究的实验设计、执行以及稿件编写的主要执
行人。杨宙参与构建供体亲本材料:
Bt
材料明恢
63 (
cry1Ac
)
、
明恢
63 (
cry1C*
)
和明恢
63 (
cry2A*
)
。高冠军执行田间管理
工作。林拥军是
cry1C*
和
cry2A
基因的专利所有人。朱雪萍
和余建友参与标记选择工作。何予卿是项目的构思者及负责
人,指导实验设计,数据分析,论文写作与修改。全体作者
都阅读并同意最终的文本。
致谢
本研究由国家自然科学基金,科技部
863
计划和植物转
基因专项共同资助。
参考文献
Barton K.A., Whiteley H.R., Yang N.S., 1987,
Bacillus
thuringiensis
δ-endotoxin expressed in transgenic
Nicotiana tabacum provides resistance to lepidopteran
insects, Plant Physiol., 85: 1103-1109
Chen H., Tang W., Xu C.G, Li X.H., Lin Y.J., Zhang Q., 2005,
Transgenic
indica
rice plants harboring a synthetic cry2A*
gene of
Bacillus thuringiensis
exhibit enhanced resistance
against rice lepidopteran pests, Theor. Appl. Genet., 111:
1330-1337
Fischhoff D.A., Bowdish K.S., Perlak F.J., Marrone P.G.,
McCoormick S.M., Niedermeyer J.G., Dean D.A., Kusano
K.K., Mayer E.J., Rochester D.E., Rogers S.G., Fraley
R.T., 1987, Insect tolerant transgenic tomato plants,
BioTechnology, 5: 807-813
Gazit E., la Rocca P., Sansom M.S.P, Shai Y., 1998, The
structure and organization within the membrane of the
helices composing the pore-forming domain of
Bacillus
thuringiensis
δ-endotoxin are consistent with an
“unbrella-like” structure of the pore, Proc. Natl. Acad. Sci.
USA, 95: 12289-12294
Hofmann C., Luthy P., Hutter R., Pliska V., 1988b, Bingding of
the delta-edotoxin from
Bacillus thuringiensis
to
brush-border membrane vesicles of the cabbage butterfly
(
Pieris brassicae
), Eur. J. Biochem., 173: 85-91
Hofmann C., Vanderbruggen H., Hofte H., Van Rie J., Jansens
S., Van Mellaert H., 1988a, Sepcificity of
Bacillus
thuringensis
δ-endotoxins is correlated with the presence
of high-affinity binding sites in brush-border membrane of
target insect midgets, Proc. Natl. Acad. Sci., USA, 85:
7844-7848
James C., 2005, Global status of commercialized biotech/GM
Crops: ISAAA
Knowles B.H., 1994, Mechanism of action of
Bacillus
thuringiensis
insecticidal proteins, Adv Insect Physiol, 24:
275-308
Martin P.A.W., Travers T.S., 1989, Worldwide abundance and
distribution of BT isolates, Appl. Environ. Microbiol., 55:
2437-2442
Murray M.G., Thompson W.F., 1980, Rapid isolation of high
molecular weight plant DNA, Nucleic. Acids. Res., 8:
4321-4325
Schwartz J.L., Juteau M., Grochulski P., Cygler M., Prefontaine
G., Brousseau R., Masson L., 1997, Restriction of
intramolecular movements within the Cry1Aa toxin
molecules of Bacillus thuringensis through disulfide bod
engineering, FEBS Lett., 410: 397-402
Schnepf E., Crickmore N., Van Rie J., Lereclus D., baum J.,
Feitelson J., Zeigler D.R., Dean D.H., 1998, Bacillus
thuringiensis and its pesticidal crystal protein, Microbial
Mol. Biol. Rev., 62: 775-806
Vaeck M., Reynaerts A., Höfte H., Jansens S., Beukeleer M.D.,
Dean C., Zabeau M., Montagu M.V., Leemans J., 1987,
Transgenic plants protected from insect attack, Nature,
328: 33-37
Van Rie J., Jansens S., Hofte H., Degheele D., Van Mellaert H.,
1989, Specificity of
Bacillus thuringiensis
delta-endotoxins.
Importance of specific receptors on the brush border
membrane of the mid-gut of target insects, Eur. J. Biochem.,
186: 239-247
Van Rie J., McGaughey W.H., Johnson D.E., Barnett B.D.,
Van Mellaert H., 1990, Mechanism of insect resistance to
the microbial insecticide
Bacillus thuringiensis
, Science,
247: 72-74
Tang W., Chen H., Xu C.G., Li X.H., Lin Y.J., Zhang Q.F., 2006,
Development of insect-resistant transgenic
indica
rice witha
synthetic
cry1C*
gene, Mol. Breeding, 13: 301-312
Zhang Q.F., 2007, Strategies for developing Green Super Rice,
Proc. Natl. Acad. Sci., USA, 104: 16402-16409