Molecular Plant Breeding 2015, Vol.6, No.18, 1
-
8
7
segregating sites (Watterson 1975) were calculated.
Tajima’s
D
(1989), Fu and Li’s (1993) and Fu’s Fs
(1997) neutrality tests were conducted by using
LDnaSP v. 4.0. Tajima’s D test was calculated for each
locus and reflects the difference between π and θ
W
. Fu
and Li’s and Fu’s Fs tests were computed without
outgroup.
Dendrograms were constructed based on the distance
matrix with the UPGMA method using the MEGA
program (Version 4, Tamura et al. 2007). Bootstrap
(1000 replications) analysis was performed to estimate
the confidence of the topology of the consensus tree.
4 Acknowledgements
This project was funded by the Technical Cooperation
Project on Korea-Africa Food and Agricultural
Cooperation Initiative between the Rural Development
Administration of the Republic of Korea and the
Republic of Tunisia. We are grateful to Dr I.S. Yoon
and Dr M.O. Byun for providing technical help during
the research.
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