Molecular Plant Breeding 2015, Vol.6, No.18, 1
-
8
6
Table 1 List of Tunisian olive cultivars included in this study
Denomination variety
Origin of production
Status
Annual amount of precipitation
a
(mm)
Barouni
North
Sensitive
500 <P< 1000
Bidh-Hmam
South
Sensitive
P< 200
Chemchali
South
Resistant
P< 200
Chamlali jerba
South
Resistant
P< 200
Chamlali Sfax
South
Resistant
P< 200
Chétoui
North
Sensitive
500 <P< 1000
Dhokar Tataouine
South
Resistant
P< 200
Fokhari
South
Resistant
P< 200
Jerboui
North
Sensitive
500 <P< 1000
Ouislati
Center-North
Medium resistant
200 <P< 400
Meski
Center-North
Medium resistant
200 <P< 400
Neb Jamel
North
Sensitive
500 <P< 1000
Toffehi
South
Resistant
P< 200
Zalmati
South
Resistant
P< 200
Zarrazi Zarzis
South
Resistant
P< 200
a Provided in:
Table 2 Genetic diversity parameters and neutrality tests for the two studied genes
TIP
(
accession N°:
DQ202710.1
)
PIP2
(accession N°: DQ202708.1)
Primer
F : CTT AAG TTT GCC ACT GGT GGA
CTC G
R : GGT AGG GAG CTG TTG ATG GCT
CTG
F : GCT GGC ATA GCA GAG TTC ATA
GCC
R : CTA CGT TTG GCA TCA GTA GCA
GAG
Annealing temperature (°c)
60
60
Total length sequenced (bp)
519
1064
Coding region (bp)
350
42
Non-coding region (bp)
169
1022
Coding region SNP number
45
8
Non-coding region SNP number
9
57
Average frequency of SNP (bp/SNP)
9.80
16.8
Total Nucleotide diversity (π x 10
-3
)
0.0024
0.0017
ϴ
w
(π x 10
-3
)
0.0020
0.0013
Non-coding sites (π x 10
-3
)
0.0024
0.0016
Coding sites (π x 10
-3
)
0.0024
0.0019
Syn. Sites (π x 10
-3
)
0.0021
0.0030
Non-syn. Sites (π x 10
-3
)
0.0024
0.0031
% Observed Homozygosity
65.0
55.0
% Expected Heterozygosity
24.3
18.7
Tajima’s
D
T
0.560
0.33
Fu and Li’s
F
0.598
0.498
Fu’s
Fs
1,068
-0,129
