MPB-2015v6n17 - page 27

Molecular Plant Breeding 2015, Vol.6, No.17, 1-22
http://mpb.biopublisher.ca
19
Continuing Appendix 3
S/N Germplasm Entry Name Group based on
Euclidean distance
From PowerMarker
Group based
on Structure
at K=2
Group based
on Structure
at K=3
Group based
on Structure
at K=4
Group based
on Structure
at K=5
163 Hybrid 5062 CKIR09001 SBR hybrids Pop1 Pop2 Pop2 Pop1
164 Hybrid 5063 PH4 SBR hybrids Pop1 Pop2 Pop2 Pop1
165 Hybrid 5064 CKIR09007 CH susceptible Pop1 Pop2 Pop2 Pop1
166 Hybrid 5065 H626 CH susceptible Pop1 Pop2 Pop2 Pop1
167 Hybrid 5066 PH1 CH susceptible Pop1 Pop2 Pop2 Pop1
168 Hybrid 5067 CKPH08003 SPR hybrids Pop2 Pop3 Pop4 Pop3
169 Hybrid 5068 H513 CH susceptible Pop1 Pop2 Pop2 Pop1
170 Hybrid 5069 CKIR09004 SBR hybrids Pop1 Pop2 Pop2 Pop1
171 Hybrid 5070 CKIR07004 SBR hybrids Pop1 Pop2 Pop2 Mixed
172 Hybrid 5071 CKIR07017 SPR hybrids Pop1 Mixed Pop2 Pop1
173 Hybrid 5072 CKIR06004 SBR hybrids Pop1 Pop2 Pop2 Pop1
174 Hybrid 5073 KH600-15A CH susceptible Pop1 Pop2 Pop2 Pop1
175 Hybrid 5074 SCDuma41 SPR and other lines Pop1 Mixed Pop1 Pop4
176 Hybrid 5075 500Q SPR hybrids Pop1 Mixed Mixed Mixed
177 Hybrid 5076 CKPH08002 SPR hybrids Pop2 Pop3 Pop4 Pop3
178 Hybrid 5077 CKIR06007 SBR hybrids Pop1 Pop2 Pop2 Pop1
179 Hybrid 5078 CKPH08041 SPR hybrids Pop2 Pop3 Pop4 Pop3
180 Hybrid 5079 DH04 CH susceptible Pop1 Pop2 Pop2 Pop1
181 Hybrid 5080 DH02 CH susceptible Pop1 Pop2 Pop2 Pop1
182 Hybrid 5081 CKIR07002 SBR hybrids Pop1 Pop2 Pop2 Pop1
183 Hybrid 5274 CKIR04003 SBR hybrids Pop1 Pop2 Mixed Mixed
184 Hybrid 5280 CKIR04002 SBR hybrids Pop1 Pop2 Pop2 Pop1
was divided into two equal parts, one section for 10
stem borer infested plants while the other portion of
10 plants was protected from stem borer damage by
applying bulldock® 25 EC insecticide at a
concentration of 25 g/l Beta-Cyfluthrin and acted as
the control. Stem borer infestation was done approximately
3 weeks after planting by artificially infesting the 10
plants per plot with 5 first-instar neonates of C.
partellus in Kiboko and B. fusca in Embu. The stem
borers larvae used in this experiment were obtained
from KARI-Katumani insectary. Leaf-damage for
each individual plant was scored two weeks after
infestation on a scale of 1 to 9 (1= no visible leaf
damage; 9= plants dying as a result of leaf damage)
(Tefera et al., 2011). At harvest, the numbers of exit
holes on the stems were counted and the cumulative
tunnel length was measured by splitting the stems.
Ears from stem borer uninfested plots were harvested,
sun-dried to a moisture content of 12-13 % and used
for, maize weevil and larger grain borer evaluation at
the KARI/CIMMYT Entomology Laboratory in
Kiboko as described below.
3.2 Evaluation for maize weevil and larger grain
borer
The maize grains were disinfested by fumigating with
phostoxin tablets for seven days to eliminate field
infestation. For each genotype 100 grams of grain
from each plot per replication was placed in 250 ml
jars, infested with 50 unsexed 7-10 day old maize
weevils and larger grain borer separately, and stored
for 90 days at a temperature of 26-28 ºC and relative
humidity of 70-75 %. The insects used in the
experiment were obtained from the KARI/CIMMYT
Kiboko maize Entomology Laboratory where they
were reared on the grains of maize cultivar PH3253
under controlled conditions (28ºC and 75% relative
humidity). Evaluation was conducted using a
completely randomized design with 3 replications.