Molecular Plant Breeding 2015, Vol.6, No.17, 1
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Continuing Appendix 2
Genotype and group name
CKSPL10218=SPRandotherlines CKSBL10016=SBR-lines
CKIR04002=SBR-hybrids
CKSPL10113=SPRandotherlines CKSBL10060=SBR-lines
CKIR04003=SBR-hybrids
CKSPL10111=SPRandotherlines CKSBL10038=NA
CZL00003=SBR-hybrids
CKSPL10112=SPRandotherlines CKSBL10033=NA
CKIR07003=SBR-hybrids
CKSPL10170=SPRandotherlines CKSBL10042=NA
CML395=SBR-hybrids
CKSPL10177=SPRandotherlines CKIR07009=NA
CKIR07008=SBR-hybrids
LaPosta-50=SPRandotherlines
PH3253=NA
CKIR07002=SBR-hybrids
LPSC7-52=SPRandotherlines
CML144=SPR-hybrids
CML395-CML444=SBR-hybrids
CML254=SPRandotherlines
500Q=SPR-hybrids
CKIR06001=SBR-hybrids
P100C-54=SPRandotherlines
631Q=SPR-hybrids
CKIR09002=SBR-hybrids
CML441=SPRandotherlines
SCDuma43=SPR-hybrids
CKIR06009=SBR-hybrids
CML443=SPRandotherlines
SCSimba61=SPR-hybrids
PH4=SBR-hybrids
CZL01005=SPRandotherlines
CML445=SPR-hybrids
CKIR09008=SBR-hybrids
CKSBL10046=SPRandotherlines CKIR07017=SPR-hybrids
CML202-CML204=SBR-hybrids
CKSBL10043=SPRandotherlines 531A=SPR-hybrids
CKIR09001=SBR-hybrids
CML159=SPRandotherlines
533A=SPR-hybrids
CKIR06007=SBR-hybrids
CZL03014=SPRandotherlines
WH403=SPR-hybrids
CKIR09006=SBR-hybrids
LPSC7-51=SPRandotherlines
CML204=SPR-hybrids
CKIR09005=SBR-hybrids
Using the biophysical/bioassay traits which are
adaptive, it was possible to discriminate the resistant
from the susceptible but not according to their
pedigree. The integrated analysis using SSR markers
suggested that the maize germplasm was likely to be
composed of four subpopulations (k = 3), one group
of storage pest resistance lines, another group of stem
borer resistance lines related to stem borer resistant
hybrids, a third group of storage pest resistant hybrids
and a fourth group constituting commercial hybrids
from different seed companies within Kenya and a
mixed group formed by the remaining genotypes. The
grouping based on the SSR markers was highly
consistent with the pedigree data. The results of this
study can be directly used by breeding programs to
better explore the genetic variability within the groups
to develop new lines and between the groups to
generate hybrids resistant to both field and postharvest
insect pests in maize.
3 Materials and Methods
3.1 Evaluation for maize stem borer
A total of one hundred eighty four maize genotypes
comprising of 100 inbred lines and 84 hybrids, from
CIMMYT Kenya selected from CIMMYT Kenya
breeding program was used in the study (Appendix 2).
All the 184 genotypes and 36 checks (20 stem borer
resistant and 16 susceptible checks) were evaluated
for
Chilo partellus
and
Busseola fusca
resistance in
October 2010 and April 2011 at the Kenyan
agricultural research institute (KARI) stations both in
Kiboko and Embu, Kenya. Kiboko is a dry and
mid-altitude agro-ecological zone located at an
elevation of 975 meters above sea level (masl), 37°
75´E and 2°15´S. it has a sandy clay soil with an
average annual rainfall of 530 mm and a mean
minimum and maximum temperature of 14.3 and
35.1
℃
, respectively. Embu is a moist and mid-altitude
zone located at an elevation of 1350 masl, and 37º42´
E and 0º49´S. Embu has a clay loam soil with an
annual rainfall of 1,200 mm and a mean minimum and
maximum temperature of 14.1 and 25
℃
, respectively.
Trials were planted in two-row plots of 5 m long at
0.25 m between hills and 0.75 m between rows using
an alpha lattice design, with three replications per
location. Two seeds were planted per hill and later
thinned to one, giving a total plant density of 53,333
plants per hectare. In order to ensure a healthy crop,
agronomic practices including weeding, fertilizer
application and supplemental irrigation were done
according to good agricultural practices. Each plot
