Molecular Microbiology Research 2015, Vol.5, No.5, 1-3
3
Junagadh Agricultural University, Junagadh, Gujarat,
India. The affected quarter milk was sampled
aseptically and transported immediately at 4°C to the
laboratory. A heavy inoculum of thoroughly mixed
mastitis milk was inoculated on 5% Ox Blood Agar
(OBA) and Sabouraud Dextrose Agar (SDA) and
incubated at 37°C for 24-48 h and at room
temperature for 2-7 days, respectively. The inoculum
inoculated of SDA were incubated at room
temperature and examined for growth every day (24,
48, 72 hrs up to week) after which plates showing no
growth were considered as negative (Tarfarosh and
Purohit, 2008). The fungal colonies were studied for
their cultural and morphological characteristics. The
morphological characteristics were noted after
staining with Gram’s and lacto phenol cotton blue
stain. Colonies obtained on OBA were transferred to
MacConkey Agar (MA), Eosin Methyline Blue Agar
(EMB) and Nutrient Agar (NA) for further
identification. Colonies were identified morphologically
by Grams staining and Biochemical identification was
done using 3% Potassium Hydroxide (3% KOH),
catalase and oxidase test as per the methods described
by Carter (1994) and Cowan and Steel (1974) (result
not shown in this article).
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