Molecular Microbiology Research 2015, Vol.5, No.5, 1-3
2
Figure 1 white or yellowish, cottony colonies having resemblance
with
Candida spp
.
Aspergillus spp.
(Figure 2) and were reveled non-septate
conidiophore with black spore heads.
3 Discussion
The incidence of mycotic infection in clinical cases of
mastitis was 13.64% (6). Similarly, the same incidence
rate of fungal mastitis (13%) were reported by
Sukumar and James, 2012 and higher rates of
incidence 29.27% and 34% were reported by Simaria
and Dholakia, 1986 and Sudarwanto, 1987, respectively.
The incidence of mastitis due to fungi have also been
reported by various workers (Zaragoza et al., 2011;
Ranjan et al., 2011; Lagneau et al., 1996; Singh et al.,
1992; Shah et al., 1986; Sharma et al., 1977). Out of 6
isolates of fungi, 2 isolates yielded smooth cottony
colonies withbudding cells resemblance with
Candida
spp.
of fungi as described by Pachauri, et al., 2013.
Similarly, Sukumar and James (2012), (Gupta et al.,
1981) and Simaria and Dholakia (1986), reported
relatively more frequent isolation of
Candida
tropicalis
from mastitis udder than any other species
of Candida. Remaining 4 isolates having greenish or
black pigmentation colonies with non-septate
conidiophore resemblance with
Aspergillus spp.
which were also described by Pachauri, et al., 2013.
Other researcher have also reported
Aspergillus spp
.
as a one of the causative agent for bovine mastitis
(Blowey and Edmondson, 2010; Stephan et al., 2000)
The incidence of fungal mastitis in this study may be
due to unhygienic condition of the animal sheds and
high humidity along with favorable environmental
conditions supporting growth of fungal spores.
Thereby that increases the chances of fungal spore to
enter into the udder which provide suitable
environment to these fungi for their growth (Elad et al.,
1995; Williamson and Di Menna, 2007). Second probable
Figure 2 Greenish or black pigmentation, showing resemblance
with
Aspergillus spp.
cause is extensive and indiscriminate use of antibiotics
for treatment of mastitis, because some of the samples
during the study were collected from the mastitic
cases, which had been unresponsive to antibiotics
treatment.
According to Loftsgard and Lindquist (1960),
Candida spp.
utilizes nitrogen from penicillin and
tetracycline antibiotics. The use of such antibiotics
encourages establishment of the infection by
damaging the mammary epithelium. Similarly, during
this study, it was observed that initially, the use of
antibiotics worsened the condition of clinical mastitis.
Chahota et al., (2001) documented that association of
fungi (
G. candidum)
with mastitis is greater in those
patients which have been subjected to prolonged
irrational antibiotic therapy, as is evident in this case.
The incidence of mycotic mastitis may be due to
development of antibiotic resistance in the bacteria
which prolongs treatment and favoring growth of
fungal spp. such as
Candida
and
Aspergillus
to infect
as secondary invader, due to physiological change in
udder and milk. The managemental practices adopted
at Cattle Breeding farm, like discarding first few strips
of milk on ground while milking of animals as well as
during treatment of mastitic animals and reluctant to
disinfect hand between milking by milkers may
contributes as potent source of lateral transmission of
infection in farm. The same were reported by Pachauri
et al., 2013. Further detailed investigation is required
in relation to pattern of antibiotic therapy advocated as
well as managemental and hygienic practices of farm
animals and workers.
4 Materials and Methods
Forty four milk samples were collected from cows
showing clinical mastitis from cattle Breeding Farm,
