International Journal of Marine Science 2015, Vol.5, No.53: 1-6
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Table 1 Methanol Extract Antibacterial Test Result from
Halimeda
sp (48 hours)
No.
Material
S.Typhi
(-)
S.aureus
(+)
E.coli
(-)
B.subtilis
(+)
1
Halimeda opuntia
12 mm
4 mm
15 mm
7 mm
2
Halimeda gracillis
10 mm
12 mm
-
11 mm
3
Halimeda renschi
11 mm
4 mm
-
13 mm
4
Halimeda macrophysa
3 mm
8 mm
13
4 mm
Table 2 Result Test of Extract Phytochemicals of
H.renshi
and
H. gracillis
No
Phytochemicals Test
Phytochemicals Test Analysis Result
Method
H. gracillis
H. renschi
1.
Alkaloid
Negative
Negative
Qualitative Analysis
2.
Steroid
Positive
Positive
3.
Terpenoid
Negative
Negative
4.
Tanin
Negative
Negative
5.
Saponin
Positive
Positive
6.
Falavonoid
Negative
Negative
KLT Test Result
Furthermore, each extract was analyzed with TLC
plate, TLC Results showed suspected stain patterns
potentially contain secondary metabolites with the
invisibility of the dominant pattern fluorescent stain
under UV light but the compound is not pure and there
are still impurities. Isolation and purification are still
needed to determine the type of the active compound
(Figure 1).
The test results on the four (4) types of
Halimeda
sp are
extracted on the growth of
E. coli
bacteria showed that
extracts of
H.opuntia
and
H. macrophysa
which has a
significant influence with a diameter of between 13-15
mm. This shows that the extract has a Halimeda extract
inhibition against the bacteria E. coli. While
H.gracillis
and
H.renschi
no effect. The pattern of growth can be
seen in Figure 2.
To test the growth of
S. aureus
on the four
Halimeda
sp seaweed extract shows have influence with a
diameter of 4-12 mm. The largest to the smallest
diameter is the extract of
H. gracillis, H. macrophysa,
H. renschi,
and
H. Opuntia
. This means that all
sample extracts have the ability/inhibition of the
growth of bacteria. The highest inhibition owned by
extracts of seaweed species
H. gracillis
with 12 mm.
While the lowest inhibitory owned by
H. renschi
and
H. opuntia
with inhibition of 4 mm. The pattern of
growth can be seen in Figure 3.
Halimeda
sp extract test results against bacterial
growth related
B. subtilis
shows that extracts of Halimeda
have influence with diameter about 4-13 mm. The
highest inhibition by 13 mm at
H.renschi
extracts,
whereas inhibition of the lowest in the extract of
H.
macrophysa
with a diameter of 4 mm (see Figure 4).
The fourth extract Halimeda also tested for bacterial
growth S.typhi. The test results showed that the
extract had an influence with a diameter of 3-12 mm.
The most high-power inhibitor is owned by
H. opuntia
Figure 1 KLT test result
Figure 2 Results of Antibacterial Test Methanol Extracts of the
E. coli
bacteria on a 48 hours observation (A.
H.opuntia
, B
H.gracillis
, C.
H renschi
and D.
H macrophysa
)
