International Journal of Horticulture 2015, Vol.5, No.9, 1
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M: Molar mass of 465.2 g/mole
ε:molar extinction coefficient of 29 000
DF: dilution factor(the total volume of extract- buffer
solution divided by the volume of extract).
V: The volume of acidic methanol solution in ml used
in the extraction.
m: the mass of sepals (mg).
The amount of total Aluminum:
It was measured in
the leaves and the flowers as the following (Jones,
2001) .Weigh 2 g of planting sample in a porcelain pot
which it was weighed when it was empty then take the
weight of all ( planting sample + porcelain pot).
Put the full porcelain pot in the ashing instrument for
two hours at 550ْc.
Chill the samples, weigh them and calculate the percent
of ashing.
Move the ashing to a flask (100 ml) then add 3 ml of
Hcl 25% and 50 ml of distilled water and heat them for
one hour.
The samples were measured by the atomic absorption
instrument .
Results and Discussion
Results in tables 1, 2, 3, 4 and also in figures 1, 2, 3, 4,
indicate that the content of chlorophyll a, b and
Carotenoids increased by adding some concentrations
of aluminum to the irrigation water. This was true in
the two studied cultivars, however the cultivar Nikko
blue recorded the highest values of them (1.33, 2.48,
1.14). The results showed that the second treatment
was the best treatment in the two cultivars with
significant effects in the above mentioned parameters
(1.69, 2.80, 2.48). It was noticed that when the
concentration of aluminum increased more than 50
mg/l, the chlorophyll a, chlorophyll b, Carotenoids
and anthocyanin content were decreased in the two
cultivars. The results also showed that the cultivar
Nikko Blue have chlorophyll a, b and Carotenoids
content more than the cultivar Pia in all treatments,
and recorded the highest values of the previous
parameters under aluminum concentration of 50 mg/L
(1.83, 3.05, 2.63). But the anthocyanin content in
sepals was more in the cultivar Pia, it was recorded in
the second treatment 50 mg/L (0.63 my/l) while it was
recorded in Nikko Blue (0.20 mg/l)
and it was
decreased when the concentration of Aluminum was
increased to more than 50 mg/l in the two cultivars.
The results also showed that the amount of total
aluminum increased in leaves and flowers when the
Table 1 Effect of Aluminum on the content of chlorophyll a in
the studied cultivars of
Hydrangea
at complete flowering stage
(µg/ml)
Treatment
Nikko blue
Pia
Mean
control
1.83
1.33
1.37
b
Al (50 mg/L)
1.83
1.55
1.69
a
Al (100 mg/L)
1.19
0.99
1.09
c
Al (150 mg/L)
0.90
0.78
0.84
d
Mean
1.33
a
1.16
b
1.25
SD
0.34
0.30
0.33
SE
0.04
0.04
0.03
Variables
cultivars
treatment
interaction
LSD 0.05
0.08
0.11
1.15
Different letters indicate that mean difference between
treatments are significant at 0.05 level in the same column and
between cultivars are significant at 0.05 level in the same row
Table 2 Effect of Aluminum on the content of chlorophyll b in
the studied cultivars of
Hydrangea
at complete flowering stage
(µg/ml)
Treatment
Nikko blue
Pia
Mean
control
2.58
2.32
2.45
b
Al (50 mg/l)
3.05
2.55
2.80
a
Al (100 mg/l)
2.26
2.00
2.13
c
Al (150 mg/l)
2.03
1.93
1.98
d
Mean
2.48
a
2.20
b
2.34
SD
0.39
0.25
0.35
SE
0.05
0.03
0.03
Variables
cultivars
treatment
interaction
LSD 0.05
0.08
0.11
0.16
Different letters indicate that mean difference between
treatments are significant at 0.05 level in the same column and
between cultivars are significant at 0.05 level in the same row
Table 3 Effect of Aluminum on content of Carotenoids in the
studied cultivars of
Hydrangea
at complete flowering stage
(µg/ml)
Treatment
Nikko blue
Pia
Mean
control
0.77
0.56
0.67
b
Al (50 mg/l)
2.63
2.33
2.48
a
Al (100 mg/l)
0.59
0.44
0.52
b
Al (150 mg/l)
0.55
0.22
0.39
b
Mean
1.14
a
0.89
b
1.02
SD
0.87
0.85
0.87
SE
0.10
0.10
0.07
Variables
cultivars
treatment
interaction
LSD 0.05
0.20
0.29
0.41
Different letters indicate that mean difference between
treatments are significant at 0.05 level in the same column and
between cultivars are significant at 0.05 level in the same row
