Bt Research 2015, Vol.6, No.9, 1-4
2
5,287,484 bp and three circular plasmids, which were
named S2685-1P01 (151,717 bp), S2685-1P02
(196,775 bp) and S2685-1P03 (49,527 bp), respectively
(Figure 1).
The chromosome encodes 6326 predicted open reading
frames (ORFs) including 5429 coding sequences
(CDSs) and the total length of genes was 4,462,815 bp,
which makes up 84.4% of genome. The G+C content
of chromosome was 35.08% and 53 tRNA and 5
rRNA operons were predicted. Approximately
，
2233
CDSs were annotated into the 189 pathwaysby using
KAAS (Moriya et al., 2007). These plasmids contain a
total of 533 predicted ORFs. The G+C contents range
from 31.86% to 34.89% (Table 1).
Two crystal genes have been predicted from this strain,
and they located on plasmid S2685-1P01 and
S2685-1P02, respectively. Strangely, this two crystal
genes share 100% sequence identity to
cry1Ac
, which
has been identified in
B. thuringiensis
serovar kurstaki
str. HD73. Based on the phylogenetic trees from the
chromosome, strain
B. thuringiensis
S2685-1 was
found to be closest to
B. thuringiensis
serovar kurstaki
str. HD73 (Song et al., 2013).
In brief, the genome sequence of S2685-1 strain not
only enriches the genome database of
B. thuringiensis
,
but also facilitates understanding the function of toxic
proteins against
Helicoverpa zea
.
Nucleotide sequence accession
The draft genome sequence of Strain S2685-1 has not
been yet included in the GenBank but deposited in the
database maintained by the HITAR Institute Canada
Inc. The annotated chromosome and plasmids would
be available upon request.
Acknowledgement
This Project of Bt Genome Sequence was initiated and
funded by the HITAR Institute Canada Inc. in British
Columbia, Canada and Guangxi Graduate Education
Innovation Projects (YCBZ2014006).
Table 1 Genome features of
Bacillus thuringiensis
S2685-1
Replicon
Total Length
(bp)
G+C content
(%)
No. of CDSs Ratio of CDSs to total length (%) rRNA operons tRNA operons
Chromosome 5,287,484
35.08
5,429
85.82
5
53
S2685-1P01 151,717
31.86
154
85.08
0
0
S2685-1P02 196,775
32.23
227
79.37
0
0
S2685-1P03 49,527
34.89
52
78.78
0
0
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