Bt Research 2015, Vol.6, No.9, 1-4
1
Research Report Open Access
Draft Genome Sequence of
Bacillus thuringiensis
Strain S2685-1, a Novel Strain
with
High Larvacidal Toxicity
against
Helicoverpa zea
Zhou Y.
1,2
, Wu Z.Q.
3,
, Liu P.P.
3
, Wei Y.J.
4
, Zhang W.F.
5
, Zhang Y.
4
, Liu S.K.
3
, Fang J.X. J.
1,2 ,3,6
1 Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, China
2 College of Life and Technology Science, Guangxi University, Nanning, Guangxi, China
3 Alkali Soil Natural Environmental Science Center(ASNESC), Northeast Forestry University, Harbin, 150040, China
4 College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China
5 College of Life Sciences, Hainan Normal University, Haikou, China
6 The HITAR Institute Canada Inc. British Columbia, Canada
*These who contributed equally
Corresponding author email
International Journal of Marine Science, 2015, Vol.6, No.9 doi
Received: 30 Oct., 2015
Accepted: 20 Nov., 2015
Published: 03 Dec., 2015
Copyright
©
2015
Zhou et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Zhou Y., Wu Z.Q., Liu P.P., Wei Y.J., Zhang W.F., Zhang Y., Liu S.K., and Fang J.X.J., 2015, Draft Genome Sequence of
Bacillus thuringiensis
Strain
S2685-1, a Novel Strain with High Larvacidal Toxicity against
Helicoverpa zea
, Bt Research, Vol.6, No.9 1
-4
(doi
Abstract
Here, we report the draft genome sequence of Bt Strain S2685-1. It consists of 262 scaffolds with a GC content of 34.89%
and a total length of 5,685,503 bp. The assemble analysis shows that there are four replicons in S2685-1, including a circular
chromosome of 5,287,484 bp with 6326 ORFs and 5429 CDSs and three circular plasmids, which are named S2685-1P01 (151,717
bp), S2685-1P02 (196,775 bp) and S2685-1P03 (49,527 bp), respectively. The average GC content of the chromosome sequence is
35.08%, while that of the plasmid sequences are 31.86%, 32.23% and 34.89%, respectively. Two crystal genes have been predicted
from this strain but they share 100% sequence identity to
cry1Ac
in HD73. It also had been identified with high larvacidal toxicity
against
Helicoverpa zea
in S2685-1.
Keywords
Bacillus thuringiensis
; S2685-1; Larvacidal Toxicity;
Helicoverpa zea
Results and Discussion
Bacillus thuringiensis
is a gram-positive entomopath-
ogenic bacteria (Broza et al., 1986), used as microbial
insecticide widely and successfully in agriculture. It
can produce lots of insecticidal parasporal crystal
proteins during sporulation (Crickmore et al., 1998).
The present studies showed that insecticidal crystal
proteins (ICPs) exhibited specific insecticidal activity
against insects belonging to the orders Lepidoptera,
Diptera, Coleoptera, Hymenoptera, Homoptera,
Orthoptera, Mallophaga, nematodes, mites and
protozoa (Schnepf et al., 1998), even were toxic to
cancer cell (Mizuki et al., 1999).
B. thuringiensis
strain
S2685-1 isolated from Heilongjiang Liangshui
National Nature Reserve belongs to the Hainan
Institute of Tropical Agricultural Resources (HITAR).
The scanning electron microscope observation showed
that it was able to generate bipyramidal parasporal
crystals and it was highly toxic to corn earworm
(
Helicoverpa zea
) (Fang et al., 2015). Here, we
present the draft genome sequence of
B. thuringiensis
strain S2685-1 and provide many valuable information of
the sequence, which will help for further understanding
the pathogenicity of
B. thuringiensis
against
Helicoverpa
zea
.
The sequencing of Bt S2685-1genome was performed
in the Shanghai Human Genome Center (CHGC,
Shanghai, China) by using an Illumina HISeq2000
Sequencing platform. The draft genome sequence
consists of 262 scaffolds with a GC content of 34.89%
and a total length of 5,685,503 bp. Gene predictions
and annotations were completed using the Glimmer
software (Delcher et al., 2007) and MetaGeneMark
software (Zhu et al., 2010). Both tRNA and rRNA
genes were identified by tRNAscan (Lowe and Eddy,
1997) and rRNAmmer (Lagesen et al., 2007 ),
respectively. Identification of the insecticidal
parasporal crystals protein gene was accomplished by
the local database comparison and DELTA-BLAST.
The draft genome of
B. thuringiensis
strain S2685-1
contains four replicons: a circular chromosome of
