Computational Molecular Biology
            
            
              12
            
            
              XJM, JM and GL analyzed the data and prepared the
            
            
              manuscript. All authors read and approved the final
            
            
              manuscript.
            
            
              
                Acknowledgments
              
            
            
              The work is funded by the Ohio Plant Biotechnology
            
            
              Consortium [grant 2011-001] (through the Ohio State
            
            
              University,
            
            
              Ohio Agricultural
            
            
              Research and
            
            
              Development Center),
            
            
              and Youngstown State
            
            
              University (YSU) Research Council [grant 2010-2011
            
            
              and 12-11]. The work is also supported by a YSU
            
            
              Research Professorship and the College of Science,
            
            
              Technology, Engineering, and Mathematics Dean’s
            
            
              reassigned time for research to XJM. JM was
            
            
              supported with a graduate research assistantship by
            
            
              the Center for Applied Chemical Biology at YSU.
            
            
              
                References
              
            
            
              Agrawal G.K., Jwa N.S., Lebrun M.H., Job D., and Rakwal R.,
            
            
              2010, Plant secretome: unlocking secrets of the secreted
            
            
              proteins, Proteomics, 10: 799-827
            
            
              http://dx.doi.org/10.1002/pmic.200900514
            
            
              Alexandersson E., Ali A., Resjö S., and Andreasson E., 2013,
            
            
              Plant secretome proteomics, Front. Plant Sci., 4: 9
            
            
              http://dx.doi.org/10.3389/fpls.2013.00009
            
            
              Bendtsen J.D., Jensen L.J., Blom N., von Heijne G., and
            
            
              Brunak S., 2004a, Feature based prediction of
            
            
              non-classical and leaderless protein secretion, Protein Eng.
            
            
              Des. Sel., 17: 349-356
            
            
              http://dx.doi.org/10.1093/protein/gzh037
            
            
              Bendtsen J.D., Nielsen H., von Heijne G., and Brunak S.,
            
            
              2004b, Improved prediction of signal peptides: SignalP
            
            
              3.0, J. Mol. Biol., 340: 783-795
            
            
              http://dx.doi.org/10.1016/j.jmb.2004.05.028
            
            
              Blum T., Briesemeister S., and Kohlbacher O., 2009,
            
            
              MultiLoc2: integrating phylogeny and Gene Ontology
            
            
              terms improves subcellular protein localization prediction,
            
            
              BMC Bioinformatics, 10: 274
            
            
              http://dx.doi.org/10.1186/1471-2105-10-274
            
            
              Borner G.H., Lilley K.S., Stevens T.J., and Dupree P., 2003,
            
            
              Identification of glycosylphosphatidylinositol-anchored
            
            
              proteins in Arabidopsis. A proteomic and genomic analysis,
            
            
              Plant Physiol., 132: 568-577
            
            
              http://dx.doi.org/10.1104/pp.103.021170
            
            
              Borner G.H., Sherrier D.J., Stevens T.J., Arkin I.T., and Dupree
            
            
              P., 2002, Prediction of glycosylphosphatidylinositol-anchored
            
            
              proteins in Arabidopsis. A genomic analysis, Plant
            
            
              Physiol., 129: 486-499
            
            
              http://dx.doi.org/10.1104/pp.010884
            
            
              Boudart G., Minic Z., Albenne C., Canut H., Jamet E., and
            
            
              Pont-Lezica R., 2007, Cell wall proteome, In: Samaj S.,
            
            
              and Thelen J. (eds.), Plant Proteomics, Springer,
            
            
              pp.169-185
            
            
              Bouws H., Wattenberg A., and Zorn H., 2008, Fungal
            
            
              secretomes-nature's toolbox for white biotechnology, Appl.
            
            
              Microbiol. Biotechnol., 80: 381-388
            
            
              http://dx.doi.org/10.1007/s00253-008-1572-5
            
            
              Caccia D., Dugo M., Callari M., and Bongarzone I., 2013,
            
            
              Bioinformatics tools for secretome analysis, Biochim.
            
            
              Biophys. Acta., S1570-9639
            
            
              Chen X.Y., Kim S.T., Cho W.K., Rim Y., Kim S., Kim S.W.,
            
            
              Kang K.Y., Park Z.Y., and Kim J.Y., 2009, Proteomics of
            
            
              weakly bound cell wall proteins in rice calli, J. Plant
            
            
              Physiol., 166: 675-685
            
            
              http://dx.doi.org/10.1016/j.jplph.2008.09.010
            
            
              Cheng F.Y., and Williamson J.D., 2010, Is there leaderless
            
            
              protein secretion in plants? Plant Signal Behav., 5:
            
            
              129-131
            
            
              http://dx.doi.org/10.4161/psb.5.2.10304
            
            
              Cho W.K., and Kim J.Y., 2009, Integrated analyses of the rice
            
            
              secretome, Plant Signal Behav., 4: 345-347
            
            
              http://dx.doi.org/10.4161/psb.4.4.8198
            
            
              Cho W.K., Chen X.Y., Chu H., Rim Y., Kim S., Kim S.T., Kim
            
            
              S.W., Park Z.Y., and Kim J.Y., 2009, Proteomic analysis
            
            
              of the secretome of rice calli, Physiol. Plant, 135: 331-341
            
            
              http://dx.doi.org/10.1111/j.1399-3054.2008.01198.x
            
            
              Chou K.C., and Shen H.B., 2008, Cell-PLoc: a package of Web
            
            
              servers for predicting subcellular localization of proteins
            
            
              in various organisms, Nat. protoc., 3(2): 153-162
            
            
              http://dx.doi.org/10.1038/nprot.2007.494
            
            
              de Castro E., Sigrist C.J., Gattiker A., Bulliard V.,
            
            
              Langendijk-Genevaux P.S., Gasteiger E., Bairoch A., and
            
            
              Hulo N., 2006, ScanProsite: detection of PROSITE
            
            
              signature matches and ProRule-associated functional and
            
            
              structural residues in proteins, Nucleic Acids Res.,
            
            
              34(Web Server issue): W362-365
            
            
              De-la-Peña C., Badri D.V., Lei Z., Watson B.S., Brandão M.M.,
            
            
              Silva-Filho M.C., Sumner L.W., and Vivanco J.M., 2010,
            
            
              Root
            
            
              secretion of defense-related proteins is
            
            
              development-dependent and correlated with flowering
            
            
              time, J. Biol. Chem., 285: 30654-30665
            
            
              http://dx.doi.org/10.1074/jbc.M110.119040
            
            
              Ding Y., Wang J., Wang J., Stierhof Y.D., Robinson D.G., and
            
            
              Jiang L., 2012, Unconventional protein secretion, Trends
            
            
              Plant Sci., 7: 606-615
            
            
              http://dx.doi.org/10.1016/j.tplants.2012.06.004
            
            
              Duvick J., Fu A., Muppirala U., Sabharwal M., Wilkerson M.D.,
            
            
              Lawrence C.J., Lushbough C., and Brendel V., 2008,
            
            
              Computational
            
            
              Molecular Biology