MPB-2016v7n27 - page 13

Molecular Plant Breeding 2016, Vol.7, No.27, 1-9
http://mpb.biopublisher.ca
7
constructed phylogenetic tree by using the barcode technology, the different of The Lilium trnH-psbA sequences
of 67 germplasm resources were in-depth analysised as well. Which analyzed the position of 35 wild lilys (Lilium
brownii) and 32 cultivars. Also provided a theoretical basis and reference for taxonomic research and hybrids
breeding research between wild lily (Lilium brownii) and the cultivated, so that researchers can develop, utilize
and innovate to the wild lily (Lilium brownii germplasm) resources scientifically and effectively.
3 Materials and Methods
3.1 Experimental materials
Thirty-five wild lilies and thirty-two cultivars as experimental materials (Table 1) were used to extract their DNAs
from the leaves of tissue culture seedlings
Table 1 The list of lilies used for analysis of trnH-psbA DNA barcode
No. Variety name Origin No. Variety name Type
1 Lilium brownii var.viridulum Xuanen county Wanzhai township, Hubei 36 Tresor A
2 Lilium brownii Badong county Chadianzi town, Hubei 37 Apricot pixels A
3 Lilium brownii Shenlongjia songluo town, Hubei 38 Yellow pixels A
4 Lilium tigrinum Shenlongjia muyu shicaohe township town, Hubei 39 Landini A
5 Lilium tigrinum Foping county Longcaoping township, Shanxi 40 Lolly pop A
6 Lilium tigrinum Changbaishan city Erdaobaihe town, Jilin 41 Tinos A
7 Lilium tigrinum Antu county Yongqing township, Jilin 42 Loreto A
8 Lilium tigrinum Fengchen county Baoshan town, Jilin 43 Matrix A
9 Lilium tigrinum Taibai county Baiyangyuan township, Shanxi 44 Gironde A
10 Lilium leichtlinii var.maximowiezii Changbaishan mountain, Jilin 45 White piexls A
11 Lilium leichtlinii var.maximowiezii Tonghua city Zuoan township, Jilin 46 Sorpressa A
12 Lilium sulphureum Shenlongjia Hongping town, Hubai 47 Constanta O
13 Lilium sulphureum Eryuan county Sanyan town,Yunnan 48 Canberra O
14 Lilium bakerianum var. aureum Haidong county Daqingshan mountain, Yunnan 49 Corvara O
15 Lilium dauricum Antu county Yaotan forestry, Jinlin 50 Tiber O
16 Lilium dauricum Changbaishan Toudaozhan township, Jilin 51 Francia O
17 Lilium dauricum Chanbaishan Erdaobaihe town, Jilin 52 Caruso O
18 Lilium concolor var.megalanthum Antu county Yongqin township, Jilin 53 Mona lisa O
19 Lilium leucanthum Badong county Chadianzi town, Hubei 54 Energetic O
20 Lilium henryi Xuanen county Wanzhai township, Hubei 55 Sorbonne O
21 Lilium duchartrei Heqing county Niutoushan mountain, Yunnan 56 Allstar O
22 Lilium taliense Binchuan county Laishan mountain, Yunnan 57 Redlatin A
23 Lilium bakerianum var.delavayi Dali city Cangshan wutaifeng, Yunnan 58 Renoir A
24 Lilium bakerianum var.dwarf Lijiang Yulongxueshan mountain, Yunnan 59 New 59 A
25 Lilium davidii Lijiang Yulongxueshan mountain, Yunnan 60 Detroit A
26 Lilium distichum Antu Yongqin township,Jilin 61 New 61 A
27 L.distichum var.odorata Antu county Yaotan forestry, Jinlin 62 White Heaven ×Lilium
bakerianum var.delavayi
CP
28 Lilium brownii Lichuan county Baiyan town, Hubei 63 Tib ×Sorbonne CP
29 Lilium pumilum Foping county Longcaoping township, Shanxi 64 Navona×Lilium dauricum CP
30 Lilium brownii var. viridulum Foping Longcaoping township, Shanxi 65 Pollyanna×Lilium dauricum CP
31 Lilium tigrinum Qinlin mountain, Shanxi 66 Sorbonne×Lilium speciosum
var, gloriosoides
CP
32 Lilium tigrinum Taibai county Taochuan town, Shanxi 67 Avign n CP
33 Lilium davidii Dali city Xizhou town, Yunnan
34 Lilium davidii Lijiang Yulongxueshan mountain, Yunnan
35 Lilium speciosum var, gloriosoides Yongkang city, Zhejiang
Note: A : Asiatic cultivar; O: Oriental cultivar ; CP: Cross progenies
3.2 Experimental methods