Molecular Plant Breeding 2016, Vol.7, No.27, 1
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could be hybridized with wild lilies, anhuanghua Bahe (Lilium sulphureum), Ziji Baihe (Lilium leucanthum),
Meili Baihe (Lilium speciosum Thunb.) etc, which gain a lot of famous hybrids (Hu Bingfen, 2003). So, the
phylogenetic relationship of Hubei Baihe (Lilium henryi) and subgroup of Leucolirion are closer. Hubei Baihe
(Lilium henryi) and wild lilies together as a category also shows that trnH-psbA Barcode classification can be
complementary with the traditional taxonomy. At present, trnH-psbA Barcode classification has become a
mainstream technology used in taxonomy. Quan Miaohua ect.
effectively identification and classification of
Lycoris
using chloroplast trnH-psbA gene sequence to analyzed the relationship between the 16 kinds of
Lycoris
(Quan Miaohua, 2011). John etc., using Barcode technology analyzed the system relationships of 48 generas 96
species of plants, found that the correct recognition rate of
trnH-psbA
single Barcode is 83%, the correct
recognition rate of rbcL-a+trnH-psbA Combined Barcode up to 95%. Using a Barcode to reflect the system
relationship may have some limitations, but the combined Barcode can overcome this limitation to a certain extent
(John et al., 2005).
2.2 Comparison of classification between wild lilies and cultivated lilies
From the results of the phylogenetic clustering, it could be seen that the cultivated lilies of Asian lilies basically
grouped into teo sub-categories Asian lily (
Ⅰ
a) and Oriental lily (
Ⅰ
b). Asian hybrids lily also named Chaotian
lilium, which hybrid were producted by lily species with distributed in Asian area. The parents of Asian hybrids
lily included Lilium amabile (
L. amabile)
、
Lilium bulbiferum (
L. bulbiferum)
、
Lilium concolor (
L. concolor
)
、
Lilium dauricum Ker-Gawl (
L. dauricum
)
、
David lily (
L. davidii
) at least 12 specics (Zhou, 2007),
L. davidii
is
one of the original parents of Asian lilies, Asian lily
、
L. davidii
、
and Sect. Sinomartagon Comber hybridized a
subclass which was consistent with close relationship. Oriental lily mainly hybridized by petiolate group (Section
Archelirion
) with several wild lilies, and which parents included safflower lily, Lilium auratum (
L. auratum
),
Japanese lily, Lilium speciosum (
L. speciosum
) and Hubei Lily (
L. henryi
). Lilium speciosum (
L. speciosum
var.
gloriosoides
) is variant of Lilium speciosum, and is the original parents of Oriental lily. The subclass was
hybrided by oriental lily,
L. speciosum
var.
gloriosoides
and petiole group, which relationship was consistent with
L. speciosum
var.
gloriosoides
.
Most of wild lilies cluster in the second category (category
Ⅱ
), the forth category (category
Ⅳ
) and the fifth
category (category
Ⅴ
), which exhibits the feature of Intraspecific assembling and geographical aggregation. For
example, the five materials of
Lilium lancifolium Thunb
were collected from different province and group, but
which all were gathered in the
Ⅱ
a sub class’ 2
nd
branch,which also reflected the close genetic relationship within
Intraspecific different group. II B subtypes and II C subclass contained a total of 8 materials, except the one Asian
lilies, the other 7 materials were collected from Jilin Changbai mountain area. Those 7 materials were belonged to
Sect. Sinomartagon Comber, Sect. Martagon Rchb
and
Lilium dauricum
group and so on. For example, the 4
materials of V b subclass were collected from Yunnan Lijiang and Dali area, which of 3 materials were gathered
by the variant of
Lilium bakerianum Coll. et Hemsl.
The same area’s Lilium plants might be happened some
genetic exchange due to the long-term close contact.
Besides, Cultivated lilies and wild lilies (Lilium brownii) were gathered together in the first Category (Category
Ⅰ
), which indicated that the genetic relationship between them were close, and there are able to compose of
hybrid combinations as well as took a theory basis for prediction the hybridization affinity. For example, the
Ⅱ
c
subgroup 'Lolly pop' and 'White pixels' growth in Aisa with peculiar color clustered together with Baoxing lily
(
L.duchartrei
germplasm origin from Niutou Mountain, Heqing County) the Fragrance variant of lunye lily
(
L.medeoloides
) found in nature, that which indicated their genetic relationship are close, and there are able to
consist of hybrid combinations as well as make it possible to cultivate the fragrance of Aisan lily (
Lilium Asiatica
Hybrida
). In addition, they are also prospective in lilies market, such as
‘
YYellow pixels
’×
liliumlancifolium,
‘
Apricot pixels'
×
liliumlancifolium, Loreto
×
Yichang lily (
L.leucanthemum
)
2.3 Conclusion
In summary of this thesis, the Lilium trnH-psbA sequences of 67 germplasm resources were compared and
