Molecular Plant Breeding 2016, Vol.7, No.24, 1
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sunflower (Onemli, 2012), in which oil accumulation started at the beginning of seed development and ended at
seed maturation.
Table 2 Crude fat content of castor seed with different development stage
Developmental stage (d)
Crude fat content (%)
Seed
Dehusked seed
Dehusked oven-dried seed
10
0.25±0.01a
0.39±0.01a
4.69±0.02a
20
10.23±0.13b
17.15±0.37b
47.13±0.61b
30
22.23±0.04c
36.03±0.28c
60.44±0.21c
40
36.55±0.55d
52.19±0.01d
62.62±0.15d
50
46.04±0.04e
58.09±0.02e
62.93±0.05de
60
50.69±0.44f
62.89±0.23f
63.91±0.41e
Table 3 Fatty acid composition and percentage of castor seed with different development stage
Developmental stage (d)
10
20
30
40
50
60
Mytistic acid
C
1
0.60±0.03c
4
0.20±0.03b
0±0a
0±0a
0±0a
0±0a
S
2
0.002±0a
0.02±0b
0±0a
0±0a
0±0a
0±0a
A
3
0±0a
0.01±0b
0±0a
0±0a
0±0a
0±0a
Palmitic acid
C
24.30±0.23c 9.50±0.35b
1.40±0.21a
1.10±0.03a
1.20±0.03a
1.20±0.07a
S
0.06±0a
0.97±0.04e
0.30±0.05b
0.41±0.01c
0.54±0.01d
0.59±0.04d
A
0.02±0a
0.43±0.01d
0.14±0.02b
0.19±0.01c
0.20±0.01c
0.23±0.02c
Stearic acid
C
1.70±0.07b
1.20±0.01a
1.20±0.10a
1.20±0.03a
1.20±0.01a
1.20±0.06a
S
0.005±0a
0.12±0b
0.26±0.02c
0.43±0.01d
0.55±0e
0.61±0.03f
A
0.002±0a
0.05±0b
0.12±0.01c
0.21±0.01d
0.21±0d
0.23±0.01d
Oleic acid
C
21.80±0.58c 7.40±0.27b
4.80±0.24a
4.60±0.24a
4.80±0.20a
4.80±0.49a
S
0.06±0a
0.76±0.03b
1.07±0.05b
1.67±0.08c
2.19±0.09d
2.45±0.25d
A
0.02±0a
0.34±0.01b
0.51±0.02c
0.81±0.04d
0.85±0.04d
0.94±0.09d
Linoleic acid
C
31.30±0.15d 19.10±0.23c 6.50±0.15b
5.40±0.01a
5.40±0.06a
5.60±0.23a
S
0.08±0.02a
1.95±0.02c
1.44±0.03b
1.97±0.01c
2.49±0.03d
2.82±0.12e
A
0.03±0a
0.88±0.01c
0.69±0.01ab 0.96±0d
0.96±0.01d
1.08±0.04e
Linolenic acid
C
10.40±0.17c 4.10±0.03b
0.70±0.003a 0.50±0.03a
0.50±0.03a
0.50±0.03a
S
0.03±0a
0.42±0e
0.15±0.01b
0.19±0.01c
0.25±0.02d
0.27±0.02d
A
0.01±0a
0.18±0d
0.07±0.01b
0.09±0.01c
0.09±0.01c
0.11±0.01c
Arachidic acid
C
0.90±0.03b
0.20±0.03a
0.10±0
.
03a
0.10±0
.
03a
0.10±0
.
03a
0.10±0
.
03a
S
0.002±0a
0.02±0a
0.01±0.00a
0.03±0.01a
0.03±0.01a
0.03±0.01a
A
0±0a
0.01±0a
0.01±0a
0.01±0a
0.01±0a
0.01±0a
Arachidonic acid
C
0.70±0.06b
0.50±0.07ab 0.60±0.06ab 0.50±0.05a
0.50±0.03a
0.50±0.06a
S
0.002±0a
0.05±0.01a
0.13±0.01b
0.18±0.02bc 0.21±0.02cd 0.25±0.03ad
A
0±0a
0.02±0b
0.06±0.01c
0.09±0.01d
0.08±0.01d
0.10±0.01d
Behenic acid
C
1.00±0.03c
0.20±0.23b
0±0a
0±0a
0±0a
0±0a
S
0.003±0a
0.02±0b
0±0a
0±0a
0±0a
0±0a
A
0.001±0b
0.009±0c
0±0a
0±0a
0±0a
0±0a
Lignoceric acid
C
0.80±0.06c
0.20±0b
0±0a
0±0a
0±0a
0±0a
S
0.002±0b
0.02±0c
0±0a
0±0a
0±0a
0±0a
A
0.001±0b
0.01±0c
0±0a
0±0a
0±0a
0±0a
Ricinoleic acid
C
6.70±0.06a
57.00±0.42b 84.90±1.64c 86.80±0.22c 86.50±0.29c 86.10±0.95c
S
0.02±0a
5.80±0.04b
18.86±0.36c 31.71±0.08d 39.81±0.14e 43.66±0.48f
A
0.006±0a
2.62±0.02b
8.98±0.17c
15.41±0.04d 15.39±0.05d 16.76±0.18e
Note: 1: crude fatty acid; 2: seed; 3: absolute content; 4: lower case represents significant different at
P
<0.01.
By using chromatographic analysis, the fatty acid composition of castor seed was detected and included mytistic
acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic
