Molecular Plant Breeding 2016, Vol.7, No.19, 1
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9
8
collected from different parts of Bangladesh (Table 5).
2.2 Field experiment
The research work was conducted at the Regional Spices Research Centre of Bangladesh Agricultural Research
Institute (BARI) Joydebpur, Gazipur, during the period from December 2013 to May 2014. The experimental field
belongs to the Agro-ecological zone of "The Modhupur Tract", AEZ-28 (Anon., 1988). The experiment was laid
out in a Randomized Complete Block Design (RCBD) with two replications. The genotypes were randomly
allotted in each block. Each replication contained 30 genotypes having 50 cm x 50 cm spacing. The unit plot size
was 2 m length and 1m breadth. Block to block distance was 50 cm. The recommended manure and fertilizer
doses were cow dung:
10 ton/ ha, urea
:
210 kg/ ha, TSP
:
330 kg/ ha, MP :
200 kg/ ha, gypsum:
110 kg/ ha.
Necessary intercultural operations were done during the crop period for proper growth and development of the
plants. Each plot was covered with water hyacinth in the dry month to conserve the soil moisture.
2.3 Statistical analysis
Raw data were used to calculate the correlation and path analysis by Indostat 9.1 software. Genotypic and
phenotypic variance - covariance matrix with economic weight were used to analyze the expected genetic gain for
individual character and combination of characters in order to construct selection indices by the package “plant
breeding” of R-3.1.1 software according to the formula given by Singh and Chaudhary (1985).
Acknowledgement
We are thankful to Md. Matin Akand, scientific officer of Regional Spices Research Centre, Bangladesh Agricultural Research
Institute, Joydebpur, Gazipur for providing seeds of all the genotypes.
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