International Journal of Marine Science, 2016, Vol.6, No.20, 1-6
3
HPLC analysis
The extracts of each specimen were prepared quantified by HPLC. From the 1.5 ml volume of each crude extract
saved for HPLC quantification, 200 µl were transferred to a vial and the solvent removed by Speed-Vac vacuum
concentration. The obtained residue was dissolved in 500 µl acetonitrile:water 1:1 + 0.5% trifluoroacetic acid .and
10 µl injected by auto-sampling into a HPLC.
Determination of Heavy Metals
The extracts of marine Red algae
Hypnea musciformis
were then poured into auto analyzer cups and concentration
of heavy metals (Cd, Fe, Cu, Ca, Cr, Pb, Ni, Co, Mn, Zn) was determined using Atomic Absorption
Spectrophotometer (AAS). The values of the heavy metal concentrations in the extracts was calculated based on
ppm.
RESULTS
The marine Red alga samples were collected from Tuticorin coast (Fig 1). The taxonomic position of the algae
was analyzed. Fig 2 explains the detection of marine bioactive compounds from the algae
Hypnea Musciformis
using FTIR analysis Fucoidans are a class of sulfated fucose-rich polysaccharides are seen. Then carotenoid,
fucoxanthin triterpene glycosides are also observed. The methanol extracts of
Hypnea musciformis
were then
subjected to HPLC and analysis for the detection of the synthesized natural compounds. Fig 1 shows the detection
of marine derived natural products from the algae
Hypnea musciformis
like β-carotenoid and vitamin E. Then
Macroalgae-derived phytosterols can be divided into four groups, 4-desmethyl-Δ5-sterols, 4-desmetyl-Δ7-sterols,
4-methyl sterols and dihydroxylated sterols. HPLC analysis showed that the most abundant phenolic compound
was epicatechin (i.e., gallic acid, 4-hydroxybenzoic acid, catechin hydrate, epicatechin, catechin gallate,
epicatechin gallate, epigallocatechin, epigallocatechin gallate, pyrocatechol). (Figure 3 and Table 1 show the
presence pf heavy metals (Cd, Fe, Cu, Ca, Cr, Pb, Ni, Co, Mn, Zn) in the algae.
Figure 1. Alga
Hypnea musciformis
Table 1: Concentration of heavy metals in marine red alga
Hypnea musciformis
S.NO
Element
Wavelength
Fuel gas flow rate
(L/ min)
Flame type
(L/ min)
Concentration of
metals in ppm
1
Cadmium
228nm
1.8
15
0.0490
2
Iron
248nm
2.2
15
0.2768
3
Copper
324nm
2
15
0.8799
4
Calcium
422nm
2
15
30.9366
5
Chromium
357nm
2.8
15
0.5322
6
Lead
283nm
2
15
0.3643
7
Nickel
232nm
2
15
0.0242
8
Cobalt
240nm
1.6
15
0.0922
9
Manganese
279nm
2
15
2.5210
10
Zinc
213nm
2
15
1.6194
