Basic HTML Version
International Journal of Marine Science 2014, Vol.4, No.50, 1-22
http://ijms.biopublisher.ca
4
Figure 2 ETE:
Symbiodinium
gene expression.
Symbiodinium
genome copy proportion (GCP [unit-less]; A) and expression of five
genes (B-F) in
Seriatopora hystrix
samples exposed to either a control (27
℃
; hollow squares) or elevated (30
℃
; filled diamonds)
temperature for 48 h. Please see the text or Tables A1-3 for full gene names. Gene expression data were normalized as described in
the text and presented as unit-less. In all panels, error bars represent standard error of the mean (
n
=3 replicates per treatment-time).
Figure 3 ETE: host coral gene expression. Host genome copy
proportion (GCP [unit-less]; A) and expression of seven target
genes (B-H) in
Seriatopora hystrix
samples exposed to either a
control (27
℃
; hollow squares) or elevated (30
℃
; filled
diamonds) temperature for 48 h. Please see the text or Tables
A1-3 for full gene names. Gene expression data were
normalized as described in the text and presented as unit-less.
In all panels, error bars represent standard error of the mean
(
n
=3 replicates per treatment-time). In panels B and D, letters
adjacent to icons signify Tukey’s honestly significant difference
groups (
p
<0.05) for the interaction effect of time and
temperature. Expression of host coral heat shock protein-70
(
hsp70
) gene was measured by Mayfield et al. (2011).
the high temperature samples of time 24 relative to the
controls. Then, at sampling time 48, expression of this
gene was 5-fold higher in controls. Tropomyosin (
trp1
;
Figure 3C),
ezrin
(Figure 3E), phospholipase-α2
(
cplap2
; Figure 3F), organic anion transporter (
oatp
;
Figure 3G), and transient receptor cation channel (
trcc
;
Figure 3H) were, on the other hand, expressed at
similar levels between treatments across the four
sampling times (Table A2).
1.3 VTE:
Symbiodinium
gene expression
Expression of
nrt2
(Figure 4A) and heat shock
protein-70 (
hsp70
; Figure 4B) was assessed in
Symbiodinium
populations within the 24 samples, all
of which were shown to be of clade C only by
real-time PCR (C
t
<30; C
t
>35 for clade A and D
assays). Although there were no significant effects of
temperature, site of origin, or their interaction on
expression of the former (Table A3), expression of
Symbiodinium
hsp70
was significantly higher
(1.8-fold) in samples exposed to a variable
temperature regime (Table A3). The influence of the
temperature regime had a significantly greater
influence on
Symbiodinium
gene expression in
samples of the VTE (4/6 of the target genes were
significantly affected by temperature when combining
the results herein with the three genes [
psI
,
pgpase
,
and
rbcL
] assessed by Mayfield et al. [2012a].) versus
those of the ETE (0/6;
X
2
=7.6,
p
<0.01).