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International Journal of Marine Science 2014, Vol.4, No.50, 1-22
http://ijms.biopublisher.ca
3
Table 1
.
Summary of the two
Seriatopora hystrix
experiments conducted at Taiwan’s National Museum of Marine Biology and
Aquarium (NMMBA).
Although the experiments were conducted a year apart, the same aquarium system was used to conduct each
one. Also, the acclimation temperatures, which aimed to approximate
in situ
values at the time of coral collection, were slightly
different for the two experiments due to
in situ
differences at the time of coral collection.
Notes:
1
photosynthetically active radiation.
1 Results
1.1 ETE:
Symbiodinium
gene expression
Recovery of the Solaris™ spike (Thermo-Scientific)
was similar between treatments and over time (data
not shown) in samples of the ETE (Table A1), all 24
of which were found to be composed only of
Symbiodinium
of clade C (C
t
<30; C
t
>35 for clade A
and D assays).
Symbiodinium
gene expression was
normalized to both spike recovery and the
Symbiodinium
genome copy proportion (GCP;
Figure
2A); the latter of which was also found to be similar
across treatments and time (
Table A1
). Expression of
ribulose-1,5-bisphosphate carboxylase-oxygenase large
subunit (
rbcL
;
Figure 2B
), photosystem I (subunit III;
psI
;
Figure 2C
), phosphoglycolate phosphatase
(
pgpase
;
Figure 2D
), nitrate transporter-2 (
nrt2
;
Figure
2E
), and ascorbate peroxidase (
apx1
;
Figure 2F
) was
similar over time and between treatments (
Table A1
).
1.2 ETE: host coral gene expression
Expression of the eight host genes was normalized to
recovery of the Solaris spike (data not shown) and the
host GCP (Figure 3A); the latter of which was found
to be stable over time and between treatments (Table
A2). Only β-actin (
actb
;
Figure 3B
) and α-tubulin
(
tuba
; Figure 3D) were significantly affected by the
interaction of time and temperature; expression of the
former in the control nubbins increased ~4-fold from
the 24 to the 48 h sampling time (Figure 3B). In
contrast,
tuba
was expressed at 2-fold higher levels in
Experiment
Elevated temperature experiment
Variable temperature experiment
Abbreviation
ETE
VTE
Manuscript(s)
Mayfield et al. (2011)
Mayfield et al. (2012a, 2013c)
Source population(s)
(see Fig. 1.)
Houbihu (upwelling site) only
Houbihu (upwelling site)
Houwan (non-upwelling site)
Time of coral collection
in situ
June 2009
May 2010
Specimen morphology
whole colony
experimentally fragmented nubbin
Acclimation time
3 weeks
3 weeks
Acclimation temperature
27
℃
26
℃
Acclimation PAR
1
300-400 µmol m
-2
s
-1
300-400 µmol m
-2
s
-1
Time of experimentation
July 2009
June 2010
Temperature treatments
control (27
℃
)
high (30
℃
)
stable (26
℃
)
variable (23-29
℃
over a 5-hr period)
Experimental period PAR
90±10 µmol m
-2
s
-1
90±10 µmol m
-2
s
-1
Length of treatment
48 h
7 d
Biological replicates/number of
aquaria
3 aquaria/treatment x 2 treatments =
6 aquaria total
3 aquaria/treatment/site of origin (SO) x 2
treatments x 2 SO = 12 aquaria total
Technical replicates
3/tank in Mayfield et al. (2011)
1/tank herein
2/tank
Sampling times
0, 6, 12, 24, and 48 h
(time 0 samples not discussed herein)
0 and 7 d
(time 0 samples not discussed herein)
Sample size (
n
)
90 in Mayfield et al. (2011)
24 herein (see text for details.)
24 (time 7 d only) in Mayfield et al. (2012a)
24 (time 7 d only) herein
Number of gene mRNAs targeted
2 in Mayfield et al. (2011) +
12 herein = 14 total
4 in Mayfield et al. (2012a) +
10 herein = 14 total