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International Journal of Marine Science 2014, Vol.4, No.57, 1-4
http://ijms.biopublisher.ca
1
A Letter Open Access
Antibacterial Attributes of Marine Gastropod
Hemifusus pugilinus
(Born, 1778)
against Human Pathogenic Bacteria
J.Mohan Raj
1
, G.Chelladurai
1
, P.Ramasamy
1,2
, P.K Deepa
1
, M.Sasirekhamani
3
1. Department of Zoology, Kamaraj College, Tuticorin, Tamilnadu, India
2. Post- Doctoral Fellow, Institute of Marine Microbes and Ecospheres, Xiamen University, Xiamen, China
3. Centre of Advanced Studies in Botany, University of Madras, Gundy campus, Chennai ,Tamil Nadu, India
Corresponding author email
International Journal of Marine Science, 2014, Vol.4, No.57 doi: 10.5376/ijms.2014.04.0057
Received: 02 Sep., 2014
Accepted: 13 Oct., 2014
Published: 21 Oct., 2014
Copyright
©
2014 Raj et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use,
distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Raj et al., 2014, Antibacterial Attributes of Marine Gastropod
Hemifusus pugilinus
(Born, 1778) against Human Pathogenic Bacteria, International Journal of
Marine Science, Vol.4, No.57 1
-
4 (doi
Abstract
To study the antibacterial activity of marine gastropods
Hemifusus puglinius
with different solvent extracts against
clinically isolated human pathogenic bacteria. Different solvent extracts of
Hemifusus pugilinus
was screened for their activity
against
Vibrio parehaemolyticus
(J13300),
Aeromonas hydrophilla
(IDH1585),
Salmonella typhi
(C6953),
Salmonella paratyphi
A
(C6915),
Vibrio cholerae
(IDH5439) and
Escherichia coli
(H10407) using standard well diffusion method and its minimum
inhibitory concentration. The study revealed that the ethyl acetate extract of the tissues inhibited the growth of the tested pathogenic
bacterial strains. The minimum inhibitory of the tissue extract ranged from 05 to 20 mg/mL. These above observed results showed
the marine gastropods
H. pugilinus
extracts were may use as additional antibacterial compounds to inhibit the growth of harmful
human bacteria.
Keywords
Hemifusus pugilinus
; Antibacterial activity; Solvent extracts; Minimum inhibitory concentration
Introduction
Molluscs are considered as one of the most important
sources to derive bioactive compounds that exhibit
antitumor, antimicrobial, anti-inflammatory and
antioxidant activities (Anbuselvi et al., 2009).The
compounds isolated from molluscs were also used in
the treatment of rheumatoid arthritis and osteoarthritis
(Chellaram and Edward, 2009).The marine environment
is a huge source for discovering many antibacterial
drugs. Apart from the food that is derived from the
marine environment, wide varieties of antibacterial
drugs are being isolated and characterized with great
promise for the treatment of human diseases. The
mollusc extracts also exhibited antiviral and
antibacterial activity against fish pathogenic bacteria
(Defer et al., 2009).
Hemifusus pugilinus
is an
herbivorous gastropod that feeds on algae and other
flora present abundantly in the Gulf of Mannar
(Edward et al., 2008). The first attempt to the
screening of antimicrobial activity in marine
organisms was initiated around 1950’s. Since this time
large number of marine organisms from a wide range
from 1960’s to 1990’s approximately 300 bioactive
marine natural products were fields of patent
(Dhinakaran et al., 2011). Approximately 6500
bioactive compounds were isolated from marine
organisms. Among the invertebrates, the molluscs are
highly delicious seafood because of their nutritive
value next to fin fish and crustaceans. They are also a
very good source for biometrically important products
(Kamboj, 1999). Literature survey revealed the
urgent need to explore marine molluscs and not
much work has been carried out in
H. pugilinus
. In
the present investigation, we aimed to study the
antibacterial activity of tissue extracts from marine
gastropod
H.pugilinus
against clinically isolated
human pathogenicbacteria.
1 Material and Methods
1.1 Collection of species
Fresh and live animals of
H. pugilinus
were collected
from the Mandapam coastal area (Lat. 09º17΄11.3˝
Nand Long. 79º 09΄17.1˝ E), Ramanathapuram
District, Tamil Nadu, India. The collected tissues were
washed with tap water until the sand and mud were
removed from the shells. After that, the shells were